摘要
目的探讨三七总皂苷(PNS)联合氨基胍(AG)对糖尿病(DM)大鼠肾脏的保护作用及其可能机制。方法以链脲佐菌素(STZ)诱导DM大鼠模型,并随机分成DM模型组(D组)、PNS治疗组(P组)、AG治疗组(A组)和联合治疗组(P+A组),同时设立正常对照组(N组)。分别于第4、8周末应用荧光光谱法测定血清和肾皮质晚期糖基化终产物(AGEs)含量,行肾组织PAS染色测定肾小球平均截面积(MGA),并计算出肾小球平均体积(MGV)。同时检测血糖、尿量、24h尿蛋白定量、肾功能,并测量体重、肾重、肾重/体重比值等。结果 (1)P+A组体重高于D组(P<0.05),而肾重、肾重/体重比值、尿蛋白定量、CCr、血清和肾皮质AGEs、MGA和MGV均低于D组(P<0.01和P<0.05);(2)P组CCr、血清和肾皮质AGEs、MGA、MGV、8周末时肾重/体重比值及4周末时尿蛋白定量均低于D组(P<0.01和P<0.05);(3)A组CCr、血清AGEs、MGA、MGV和8周末时肾皮质AGEs均低于D组(P<0.01和P<0.05);(4)P+A组血清AGEs低于P、A组(P<0.01和P<0.05),肾皮质AGEs低于P组4周末时和A组(P<0.05),而8周末时MGA和MGV低于P、A组(P<0.05),肾重/体重比值低于A组(P<0.05)。结论 PNS和AG均能通过减少肾组织中AGEs的生成,对DM大鼠肾脏起保护作用。二者联用肾保护作用优于单一药物治疗。
Objective To investigate the renoprotective effect of treatment with panax notoginseng saponins（PNS） and aminoguanidine（AG） on streptozotocin（STZ）-induced diabetic rats as well as the possible mechanism involved in.Methods The rats diabetic model was induced by STZ.The model rats were divided into four groups：a diabetic group without treatment（group D,n=12）,a diabetic group treatment with PNS（group P,n=12）,a diabetic group treatment with AG（group A,n=12）,and a diabetic group treatment with PNS and AG（group P＋A,n=12）.And there was a normal control group（group N,n=12） as well in the experiment.Their serum and renal cortex advanced glycation end products（AGEs） contents were determined by the spectrum law of fluorescence in the 4th weekend and 8th weekend of treatment respectively.The mean glomerular area（MGA） and volume（MGV） were measured by image analysis system.The changes of the blood glucose levels,urine amount,urine albumen,the renal function,body weight（BW）,kidney weight（KW） and KW/BW ratio were observed.Results （1） Increased BW was observed in group P＋A compared with that group D（P〈0.05） and its KW,KW/BW ratio,urinary excretion of protein, creatinine clearance rate（CCr）,the content of AGEs in serun and renal cortex,MGA and MGV were significantly lower than that of group D（P〈0.01 or P〈0.05）.（2） Compared with group D,CCr,the content of AGEs in serun and renal cortex,MGA,MGV,KW/BW ratio（the end of 8 week）and urinary excretion of protein（ the end of 4 week） in group P were significantly decreased （P〈0.01 or P〈0.05）.（3）The model rats of group A had significantly lower Ccr, serun AGEs contents,MGA,MGV and renal cortex AGEs contents （the end of 8 week）than group D（P〈0.01 or P〈0.05）.（4）The content of serun AGEs,MGA and MGV（the end of 8 week）was decreased significantly in group P＋A compared with group P and group A（P〈0.01 or P〈0.05）,its the content of AGEs in renal cortex was significantly lower than that of group P（the end of 4 week）and group A （P〈0.05 respectively）,and decreased KW/BW ratio was observed in group P＋A compared with that group A（P〈0.05）.Conclusions PNS and AG both possesses renoprotective effect on STZ-induced diabetic rats,whose mechenism be associated with inhibiting the overproduction of AGEs in renal tissue.The two unite and treat,it is better at single medical herses role for kidney to protect.
出处
《中华临床医师杂志（电子版）》
CAS
2010年第4期38-43,共6页
Chinese Journal of Clinicians(Electronic Edition)
基金
浙江省医药卫生科研计划项目(2003A076)
杭州市医药卫生科研计划项目(2003A009)
