摘要
目的:基于RAPD标记开发SCAR标记,为川牛膝及其混淆品鉴定提供有效方法。方法:建立了收集于四川天全、宝兴、会理、金口河等地的19个牛膝种群(包括川牛膝及其混淆品红牛膝、怀牛膝)的RAPD指纹图谱,从中找到能有效区分上述材料的多态性谱带F300,F500进行克隆与测序;根据测序结果设计2对特异引物对19份材料进行PCR扩增。结果:引物SC-320能稳定地区分川牛膝与怀牛膝,引物SC-495则能稳定地区分川牛膝与红牛膝,2对引物结合能快速准确地鉴定川牛膝、红牛膝和怀牛膝。结论:建立了鉴定川牛膝及其混淆品的SCAR标记技术体系.
Chuanniuxi（Radix Cyathule） is one of the most important geo-herb in Sichuan province,which adulterants are Hongniuxi（Cyathula capitata） and Huainiuxi（Achyranthes bidentata）.In this paper Chuanniuxi and its adulterants were identified by SCAR markers.Nineteen populations from Tianquan,Baoxin,Huili and Jinkouhe were collected and their RAPD fingerprints were established.Based on the RAPD patterns,two polymorphic bands F300 and F500 were selected,recycled,cloned and sequenced.According to the sequences two pairs of sequence characterized amplified regions（SCAR） primers were designed and used to amplify all materials to prove the efficiency of identification of the different populations.Chuanniuxi and Huiniuxi could be distinguished by the primer SC-320,Chuanniuxi and Hongniuxi could be distinguished by the primer SC-495.Combining the two SCAR markers,Chuanniuxi,Hongniuxi and huainiuxi could be identified effectively and quickly.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第8期953-956,共4页
China Journal of Chinese Materia Medica
基金
国家科技支撑计划项目(2006BAI06A12-02)
关键词
川牛膝
混淆品
RAPD标记
SCAR标记
Radix Cyathule
promiscuous medicinal materials
RAPD marker
SCAR marker
