摘要
目的:考察人参炔醇(PAN)对实验性迟发型超敏反应的影响及可能作用途径。方法:应用2,4-二硝基氯苯(DNCB)造成小鼠变态反应性接触性皮炎(ACD)模型,考察PAN诱导相给药对耳廓肿胀的影响,并进行病理组织学检查;MTT法检测PAN对ConA诱导的T淋巴细胞增殖的影响。ELISA方法检测ConA刺激的脾细胞培养上清IFN-γ水平。结果:PAN10 mg.kg-1诱导相给药可明显抑制小鼠耳肿胀;病理组织学结果显示PAN可减轻模型小鼠局部水肿及血管扩张,减少淋巴细胞浸润;PAN对ConA诱导的T细胞增殖有明显的抑制作用,而对静息状态的脾细胞未观察到显著影响;PAN10μmol.L-1起明显抑制体外ConA刺激脾细胞的IFN-γ分泌,且作用在早期(6 h)即已呈现。结论:PAN对实验性迟发型超敏反应有显著的抑制作用,该作用可能与抑制T细胞增殖和IFN-γ的分泌有关。
Objective： To evaluate the effect of panaxynol（PAN） on delayed type hypersensitivity and possible mechanism.Method： Allergic contact dermatitis（ACD） was induced by DNCB as a delayed type hypersensitivity（DTH） model to observe effect of PAN on auricle inflammation including pathological injury.Proliferation of T lymphocytes was induced by ConA and measured by MTT method.IFN-γ secretion of splenocyte induced by ConA was detected by ELISA.Result： The swelling degree of auricle and pathological injury in ACD mice was reduced significantly by treated with PAN in induction phase.Proliferation of T lymphocytes induced by ConA in vitro was inhibited significantly by PAN,By contrast,no detectable effect was observed in resting splenocyte.IFN-γ induced by ConA in splenocytes was inhibited markedly by PAN from 10 μmol·L-1 and from 6 h.Conclusion： The results showed that DTH was inhibited by PAN mainly in induction phase and this effect may be related with the inhibition on T lymphocytes proliferation and secretion of IFN-γ.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第8期1034-1037,共4页
China Journal of Chinese Materia Medica
基金
江苏省方剂研究重点实验室开放课题
南京中医药大学基础研究重点培育项目
