摘要
目的比较不同喂养方式对足月新生儿血清瘦素水平的影响。方法选择河北医科大学第一医院产科出生的健康足月新生儿66例,其中母乳喂养儿35例,人工喂养儿31例。在66例新生儿出生第3、7、28天采集母乳2mL于洁净试管中;于新生儿出生第7天,抽取其清晨空腹静脉血2mL,留取血清标本装洁净试管,均置于-70℃冰箱保存。配方乳采用雀巢力多精奶粉,按1匙奶粉加30mL水的比例冲调,水温为50℃。采用放射免疫法对足月新生儿血清、母乳及配方乳中瘦素水平进行测定。结果1.健康足月儿66例出生第3、7、28天母乳瘦素水平分别为(1.31±0.67)μg·L-1、(1.33±0.14)μg·L-1、(1.26±0.98)μg·L-1,不同时间母乳瘦素水平比较差异无统计学意义(F=1.78,P>0.05)。配方乳中未检测到瘦素。2.母乳喂养组新生儿出生第7天血清瘦素水平为(3.38±1.54)μg·L-1,人工喂养组新生儿出生第7天血清瘦素水平为(1.91±0.32)μg·L-1,二组血清瘦素水平比较差异有统计学意义(t=3.176,P<0.05)。结论母乳中含有一定水平的瘦素,是新生儿血中瘦素的重要来源。母乳喂养有利于维持新生儿期血中较高的瘦素水平,对新生儿生长发育及能量代谢有重要作用。
Objective To compare the effect of different feeding practices on the serum leptin levels in full term neonates.Methods Venous blood samples about 2 mL were obtained from 66 cases of 7 day-old healthy full term neonates,35 cases of them were breast-fee-ding,and 31 cases of them were formula-feeding.On the 3rd,7th,28th days of the 66 neonates after delivery,breast milk samples about 2 mL were drawn.And on the 7th day,venous blood about 2 mL were drawn in the morning at the fasting state.The serum and breast milk samples were stored at-70 ℃ refrigerator until analyzed.Formula was Nestle Latogen milk powder.The proportion was 1 spoon of powder plus 30 mL water.The temperature of the water was 50 ℃.Leptin concentrations were measured by radio-immunoassay.Results 1.Leptin levels in breast milk of 66 healthy full term neonates were （1.31±0.67） μg·L-1,（1.33±0.14） μg·L-1,（1.26±0.98） μg·L-1 on 3rd,7th,28th days after birth respectively.There was no difference between earlier breast milk and later one （F=1.78,P0.05）.Leptin was not pre-sent in formula milk samples.2.The average serum leptin concentration for the breast-feeding group was （3.38±1.54） μg·L-1 on the 7th day.The average serum leptin concentration for the formula-feeding group was （1.91±0.32） μg·L-1 on the 7th day.There was significant difference between the 2 groups（t=3.176,P0.05）.Conclusions Leptin is present in breast milk,which is the important source of the serum leptin in neonates.Breast-feeding can make higher serum leptin levels in neonates,and play an important role in growth development and energy metabolism in neonates.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第7期516-517,共2页
Journal of Applied Clinical Pediatrics
