摘要
目的研究抗凝血标本全血放置以及离心后保存温度扣时阅对血浆凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）检测结果的影响。方法取PT和APTT正常值标本180例（PT在10．5～14．0s之间，APTT在22．0～36．0s之间），异常值（高值）标本120例（PT〉14．0s，APTT〉36．0s），用SysmexCA7000型全自动血凝分析仪及其配套试剂，分剐辁洲抗凝血标本全血室温（26℃）放置及离心后在室温及冰箱（4℃）保存条件下0，2，4，6，8，12，24h时PT，APTT的结果，并将所得到的数据用统计学方法进行处理。结果全血室温放置时，正常值标本PT在4h内，APTT在2h内的测定结果与0h测定结果比较差异无统计学意义（P〉0．05）；异常值标本PT，APTT在2h内的测定结果与0h测定结果比较差异无统计学意义（P〉0．05）。离心后血浆在室温下，正常值标本PT在6h内，APTT在4h内的测定结果与0h测定结果比较差异无统计学意义（P〉0．05）；异常值标本PT，APTT在4h内的测定结果与0h测定结果比较差异无统计学意义（P〉0．05）。离心后血浆在冰箱保存条件下，正常值及异常值标本PT，APTT在6h内的测定结果与0h测定结果比较差异无统计学意义（P〉0．05）。结论凝血标本采集后，如果不能即刻测定，最好离心后置于冰箱保存，并保证在6h内完成测定；如果不离心室温放置，应在2h内完成测定。
Objective To investigate the influence of temperature and time of stored spicimens on the test results of the prothrombin time （PT）,activated partial thromboplastin time （APTT）,placed as anticoagulant whole blood as well as centrifuged. Methods Took 180 cases of normal specimens about PT and APTT （PT value was between 10.5s-14.0s and APTT value was between 22.0s-36.0s） and 120 cases of abnormal specimens （PT value was greater than 14.0s and APTT value was greater than 36.0s） and they were measured for hemostasis by Sysmex CA7000 automated blood coagulation analyzer and supporting reagent. The PT and APTT were measured immediately after the specimens were taken,at 2, 4,6,8,12 h and 24 h placed as anticoagulant whole blood at room temperature （26℃）, respectively. Meanwhile ,the specimens were measured at room temperature and refrigerator temperature（4℃） at the corresponding time after centrifuged. The results were compared by statistical method. Results When whole blood specimens were placed at room temperature, if PT of normal value were measured in 4 h,APTT in 2 h,there was no statistical difference in the results compared with measured immediately after sampled （P〉0. 05） ;as abnormal value specimens ,PT in 2 h,APTT in 2 h ,there was no statistical difference in the results compared with measured immediately after sampled （P〉0. 05）. When the specimens were placed at room temperature after centrifuged,as normal value specimens,PT in 6 h,APTT in 4 h;as abnormal value specimens,PT in 4 h, APTT in 4 h. When the specimens were placed at refrigerator temperature after centrifuged,as normal value specimens,PT in 6 h,APTT in 6 h;as abnormal value specimens,PT in 6 h,APTT in 6 h. Conclusion After the specimen for hemostasis is collected ,if not measured immediately,it＇s better to place it in the refrigerator after centrifuged and ensure that to complete the detection in 6 h. If the specimen is placed at room temperature,the detection should be completed within 2 h.
出处
《现代检验医学杂志》
CAS
2010年第2期115-117,共3页
Journal of Modern Laboratory Medicine
