期刊文献+

siRNA抑制喉鳞状细胞癌Hep-2细胞生长的作用 被引量:3

Inhibition of growth and proliferation of Hep-2 cells by targeting hTERT gene using small interference RNA technology
原文传递
导出
摘要 目的:探讨靶向人端粒酶基因hTERT对人喉鳞状细胞癌Hep-2细胞生长增殖的抑制作用。方法:根据人端粒酶基因hTERT设计和合成人端粒酶siRNA和随机的siN.C作为阴性对照,通过转染载体Lipofectamine 2000脂质体转染Hep-2细胞,进行形态学、RT-PCR和MTT法检测,观察其干扰效果。结果:①siRNA能够成功转染进入Hep-2细胞;②RT-PCR结果显示转染组与阴性对照组和空白组相比,hTERT mRNA表达明显减弱,转染组hTERT mRNA较空白组相对下降70%;③形态学和MTT结果显示人端粒酶siRNA能有效抑制Hep-2细胞增殖,抑制率达到65%。结论:靶向hTERT的siRNA能显著抑制喉鳞状细胞癌Hep-2细胞的生长增殖。 Objective:To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms.Method:siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase(RT-PCR),MTT assay,and morphology.Result:①siRNA were transfected into Hep-2 cells successfully;②RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA;③The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%.Conclusion:siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.
出处 《临床耳鼻咽喉头颈外科杂志》 CAS CSCD 北大核心 2010年第7期308-310,共3页 Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金 广东省自然科学基金资助项目(No:9451063201003299)
关键词 喉肿瘤 SIRNA 基因治疗 laryngeal neoplasms siRNA gene therapy
  • 相关文献

参考文献8

  • 1HUTVAGNER G, ZAMORE P D. RNAi : nature abhors a double-strand[J]. Curr Opin Genet Dev, 2002,12:225-32.
  • 2DZITOYEVA S,DIMITRIJEVIC N, MANEV H. Intra-abdominal injection of double-stranded RNA into anesthetized adult Drosophila triggers RNA interference in the central nervous system[J]. Mol Psychiatry, 2001,6: 665- 670.
  • 3TUSEHL T, ZAMORE P D, LEHMANN R, et al. Targeted mRNA degradation by double-stranded RNA in vitro[J]. Genes Dev, 1999,13 : 3191-3197.
  • 4陈雯 张桥 魏青 等.5-3叠氮脱氧胸腺核苷和硫代反义寡核苷酸抑制肿瘤细胞的生长以及端粒酶的活性.癌变.畸变.突变,1999,11(6):313-313.
  • 5KOLQUIST K A ,ELLISEN L W,COUNTER C M, et al. Expression of TERT in early premalignant lesions and a subset of cells in normal tissues[J]. Nat Genet, 1998,19 : 182-186.
  • 6CHENG P Y, YUEN P W, NICHOLLS J M,et al. Telomerase activation in nasopharyngeal carcinoma [J]. Br J Cancer,1998,77:456-460.
  • 7HOHAUS S, CAVALLO S, BELLACOSA A,et al. Telomerase activity in human laryngeal squamous cell carcinoma[J]. Clin Cancer Res, 1996,2: 1896- 1900.
  • 8BERTRAND J R , POTTIER M, VEKRIS A,et al. Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo [J]. Biochem Biophys Res Commun, 2002,296 : 1000- 1004.

同被引文献39

  • 1赵家明,李明意,杨展,吴柱国,李震,张毅.脱氧核酶对鼻咽癌细胞端粒酶活性的抑制作用[J].华中科技大学学报(医学版),2005,34(3):330-333. 被引量:3
  • 2安立峰,董震.RNA干扰——肿瘤研究的新工具[J].中华肿瘤杂志,2005,27(7):385-388. 被引量:38
  • 3周俊旭,周绪红,陶泽璋,肖伯奎,陈始明,刘丹.小干扰RNA对喉鳞状细胞癌裸鼠移植瘤增殖细胞核抗原及p53蛋白表达的影响[J].临床耳鼻咽喉科杂志,2006,20(6):264-267. 被引量:2
  • 4KIRKPATRICK K L, MOKBEL K. The significance of human telomerase reverse transcriplase (hTERT) in cancer[J]. Eur J Surg Oncol,2001,27:754-760.
  • 5MILI.ER M C,LIU J K,COLLINS K. Template defi- nition by Tetrahymena telomerase reverse tran- seriptase[J]. EMBO J, 2000, 19:4412-4422.
  • 6KUMAR P, LEE S K, SHANKAR P,et al. A single siRNA suppresses fatal encephalitis induced by two different flaviviruses[J]. PLoS Med, 2006,3: e96.
  • 7GJERTSSON I, LAURIE K L, DEVITT J, et al. Tolerance induction using lentiviral gene delivery de- lays onset and severity of collagen II arthritis[J]. Mol Ther, 2009,17:632--640.
  • 8WIRTH M G,RUSSELL-EGGITT I M,CRAIG J E, et al. Aetiology of congenital and paediatric cataract in an Australian population[J]. Br J Ophthalmol, 2002, 86:782--786.
  • 9REDDY M, FRANCIS P J , BERRY V, et al. Molec ular genetic basis of inherited cataract and associated phe- notypes [J]. Surv Ophthalmol , 2004,49 : 300-- 315.
  • 10HWANG C F, SU C Y, KOU S C, et al. Diagnostic usefulness of telomerse activity in nasopharyngeal car- cinoma [J]. Cancer Res,2000 , 91:760--766.

引证文献3

二级引证文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部