摘要
目的:探讨脂多糖(LPS)对晶状体上皮细胞(LECs)Toll样受体4(TLR4)和CD14表达的影响。方法:采用不同浓度的LPS与体外培养的牛LECs共同孵育不同时间,再用一步法反转录多聚酶链反应(PCR)检测LECs中TLR4mRNA和CD14mRNA的表达。结果:50μg/LLPS组、100μg/LLPS组、200μg/LLPS组、500μg/LLPS组、1000μg/LLPS组的LECs中TLR4mRNA的表达均分别显著高于对照组(P<0.01);100μg/LLPS作用24h、48h、72h后LECs的TLR4mRNA表达均显著高于对照组(P<0.01);100μg/LLPS作用24h后LECs的CD14mRNA表达亦显著高于对照组(P<0.05)。结论:LPS可促进LECs中TLR4mRNA和CD14mRNA的表达,提示白内障手术后眼内细胞反应和后发性白内障的发生发展可能与TLR4和CD14表达增加有关。
AIM：To investigate the effect of lipopolysaccharide （LPS） on the mRNA expression of Toll-like receptor 4 （TLR4） and CD14 in lens epithelial cells （LECs）.METHODS： The cultured LECs were incubated with defferent concentrations of LPS for defferent times.The mRNA expression of TLR4 and CD14 in LECs were detected by reverse transcription polymerase chain reaction （RT-PCR）.RESULTS： The mRNA expression of TLR4 in the LECs treated with LPS at concentrations of 50 μg/L,100 μg/L,200 μg/L,500 μg/L or 1 000 μg/L was higher than that in the LECs without LPS treatment （P〈0.01）,respectively.The mRNA expression of TLR4 in the LECs incubated with 100 μg/L LPS for 24 h,48 h or 72 h was higher than that in LECs without LPS treatment at same time points （P〈0.01）.The mRNA expression of CD14 in the LECs incubated with LPS at concentration of 100 μg/L for 24 h was also higher than that in the LECs without LPS treatment （P〈0.01）.CONCLUSION： LPS enhances the mRNA expression of TLR4 and CD14 in LECs,which may be related to intraocular response,as well as to the formation and development of after-cataract.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第4期797-801,共5页
Chinese Journal of Pathophysiology
基金
福建省中医药重点资助项目(No.Wzzb0602)
