摘要
准弹性光散射技术是生物学中一种新颖的技术，具有测量速度快，精度高等 优点。准弹性光散射测量原理是基于经典的多普勒效应，利用 Ｒａｙｌｅｉｇｈ－Ｇａｎｓ－Ｄｅｂｙｅ （ＲＧＤ）近似方法推导出微粒运动速度和频率变化之间关系。对生物微粒（精子与四膜 虫）进行测量表明 ＲＧＤ近似方法完全适用。
The basic principles of light scattering which apply to the QELS technique and particle velocimetry are introduced in this paper. This is followed by a rigorous treatment of light scattering from extended particles such as biological cells.A computationally convenient form of the RGD approximation is introduced.The RGD approximation is used to treat structure and dynamic effects commonly encountered in light scattering from cells.The RGD theory is a satisfactory method for the analysis of QELS data. The motion of spermatozoa and pyriformis are measured by the QELS technique
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1990年第3期93-100,共8页
Journal of Tsinghua University(Science and Technology)
关键词
生物微粒
光散射
测量
准弹性
light scattering, Doppler effect, biological particle
