摘要
为评价青光眼滤过性手术后应用干扰素α－２ｂ预防和治疗滤过道的纤维疤痕化对眼组织的毒性作用，我们对兔眼前房内直接注入干扰素α－２ｂ以及兔眼行巩膜咬切术后滤过区旁结膜下注射药物后进行角膜组织的检查：角膜上皮用１％荧光素钠染色，角膜内皮用锥兰和茜素红进行内皮活性染色，并对全层角膜组织结构作光镜检查。结果发现：抗青光眼术后滤过区旁结膜下注射干扰素α－２ｂ５×１０５ＩＵ／０．２ｍｌ连续３～１４天，注射总量１．５×１０６ＩＵ～６×１０６ＩＵ，以及前房内直接注入干扰素α－２ｂ１×１０６ＩＵ２４小时和第１０天后，角膜上皮、内皮以及全层组织结构无变化。说明兔眼对干扰素α－２ｂ具有良好的耐受性。
n order to evaluate the corneal toxicity of adminstered interferonalpha 2b(IFN α2b) as an antifibrotic treatment after glaucoma filtering surgery,We investigated the corneal changes following subconjunctivally applied IFN α2b 5×105IU at filtering bleb for 3—14 days after sclerectomy in white rabbits and directly injection IFN α2b 1×106IU into anterior chamber.Our results showed that no sign of toxicity on corneal epithelium by application of 1% sodium fluorescein and no evidence of corneal endothelium toxicity by viability examination with dual staining of typan blue and alizanin red S.We also did not observe any histologic changes of corneal structure by light microscopy.We believed that rabbits eyes seem to be welltolerate with high doses of interferon by local used,and subconjunctivally injection IFN α2b at filtering bleb after glaucoma filtering surgery may be a safe drugapplied method.
出处
《中国实用眼科杂志》
CSCD
1998年第3期141-142,I000,共3页
Chinese Journal of Practical Ophthalmology
基金
广东省自然科学基金
关键词
干扰素
角膜毒性
青光眼
滤过性手术
Interferon corneal toxicity glaucoma filtering surgery
