摘要
结冷胶是由伊乐假单胞杆菌生成的具有重要商业价值的微生物胞外多糖。本研究为了提高伊乐假单孢菌的产结冷胶水平,采用低能氮离子注入的方法选育高产菌株。经过初筛和复筛得到一株高产结冷胶菌株Pseudomonas elodea A3-5。该菌株传代稳定,生长迅速,在7 L发酵罐内生长状态优于出发菌株,分子量和品质都较高,分批发酵结冷胶产量达到16.5g/L,较出发菌株明显提高了25%。
Gellan gum is a kind of microbial exopolysaccharides with great commercial value produced by Pseudomonas elodea. In order to increase gellan gum production, low-energy N＋ ion beam implantation was used to explore a mutant strain producing a high level of gellan gum. After screening, the high producing mutant A3-5 was isolated, which exhibited better properties and increased molecular weight. Mutant A3-5 grew much faster and subcultured stably in 7 L fermentor than the wild strain. The maximal geUan gum production in batch fermentation by A3-5 was 16.53 g/L, 2596 higher than that of the wild strain.
出处
《工业微生物》
CAS
CSCD
2010年第2期20-24,共5页
Industrial Microbiology
基金
国家高技术研究发展计划(863计划
2006AA02Z207)
国家自然科学基金(20676055)
关键词
N^+离子柬注入
结冷胶
诱变选育
产胶量
粘度
流态指数
ion implantation
gdlan gum
mutation breeding
gellan gum production
viscosity
flow index
