摘要
目的:开发一种快速测定婴儿配方乳粉中维生素A酯类含量的反相高效液相色谱法。方法:直接使用二氯甲烷和甲醇体积比为2.5:1的混合溶剂对样品进行提取,在30℃的条件下采用等度洗脱,流动相采用体积比为80:20的甲醇-三氯甲烷溶剂,流动相流速1.0mL/min;色谱柱温度30℃,检测波长325nm。结果:维生素A醋酸酯和维生素A棕榈酸酯的回收率分别为100.1%和102.8%,多次测定结果的相对偏差分别为1.18%和0.96%,检出限分别为3.1ng和4.3ng。结论:本方法操作简单、精密度、回收率和灵敏度高,适用于婴幼儿配方乳粉中的维生素A酯类的检测。
Objective：To develop a rapid reversed-phase high performance liquid chromatographic（RP-HPLC） method for measuring retinyl esters in infant formula milk powder.Methods：Samples were directly extracted with a mixture of dichloromethane and methanol（2.5：1,V/V）.The chromatographic separation was performed at 30 ℃ in the isocratic elution mode using a mobile phase composed of a mixture of methanol and chloroform（80：20,V/V）.Results：Retinyl acetate and retinyl palmitate presented average spike recoveries of 6 replicates at 2 levels of 100.1% and 102.8%,with relative standard deviations of 1.18% and 0.96%,respectively.The detection limits for retinyl acetate and retinyl palmitate were 3.1 ng and 4.3 ng,respectively.Conclusion：The method demonstrates simplicity and high precision,recovery and sensitivity,and thus has promising potential for practical applications.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第8期154-157,共4页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAD04A15)
关键词
维生素A酯类
婴儿配方乳粉
反相高效液相色谱
retinyl esters
infant formula milk powder
reversed-phase high performance liquid chromatography
