摘要
对猪源脑心肌炎病毒(EMCV)GXLC株进行全基因组克隆、测序和分析。采用RT-PCR技术分段扩增基因组可读框(ORF),应用3'-RACE和5'-RACE技术扩增3'-UTR和5'-UTR,分别对扩增片段进行克隆和测序,经拼接后获得GXLC株全基因组序列,共7725个核苷酸。与NCBI GenBank登录的国内外参考毒株进行同源性比较及系统进化分析结果表明,GXLC株与GX0601、GX0602、BJC3、HB1等国内分离株以及CBNU、K3、K11、BEL-2887A、EMCV-R、PV21等国外分离株同源性达99%以上;基于全基因组、结构蛋白及非结构蛋白基因序列绘制的系统进化树拓扑结构图相近,所有EMCV分离株可分成两个群:I群和II群,I群可细分为Ia亚群和Ib亚群,其中猪源EMCV属于Ia或Ib亚群、鼠源EMCV属于Ia亚群、野猪源EMCV属于II群,GXLC株与其它中国分离株均属于Ia亚群。
The complete genome of encephalomyocarditis virus （EMCV）strain GXLC isolated from swine was sequenced and analyzed. Five overlapped gene fragments covering the entire open reading frame （ORF） were amplified by RT-PCR,and the 3＇-untranslated region （UTR） and 5＇-UTR were amplified by the 3＇-rapid amplification of cDNA ends （RACE） and 5＇-RACE method,respectively. The genome sequences of strain GXLC were obtained by assembling the sequences of RT-PCR-generated cDNA fragments. The length of the complete genome was 7 725 nucleotides （nt）. The homology comparison and phylogenetic analysis of the nucleotide and deduced amino acid sequences between strain GXLC and other EMCV strains available in GenBank were performed. The results showed that the complete genome identity between GXLC strain and the strains from China,i.e. GX0601,GX0602,BJC3 and HB1 and the strains from other countries,i.e. CBNU,K3,K11,TEL-2887A,EMCV-R and PV21 was over 99%. The phylogenetic trees based on the complete genome,the structural protein or the non-structural protein gene sequences revealed that the tree topology was similar. All the EMCV strains could be divided into two groups：group I and group II,and group I could be subdivided into subgroup Ia and subgroup Ib. The strains from swine belonged to subgroup Ia or Ib,and the strains from mice belonged to subgroup Ia,while the strains from Sus scrofa belonged to group II. Strain GXLC,together with other EMCV isolates from China,belonged to subgroup Ia.
出处
《病毒学报》
CAS
CSCD
北大核心
2010年第2期134-142,共9页
Chinese Journal of Virology
基金
农业部公益性行业科研专项(200803026)
广西科学基金项目(桂科青0728047)
广西科技创新能力与条件建设基金项目(08-05-01D)
关键词
猪
脑心肌炎病毒
基因组
同源性比较
系统进化分析
swine
encephalomyocarditis virus （EMCV）
genome
homology comparison
phylogenetic analysis
