摘要
目的应用酶联免疫吸附试验(ELISA)对感染后肠功能紊乱大鼠外周血Th1型细胞因子IL-1β和Th2型细胞因子IL-4的含量进行检测分析,探讨炎性因子及免疫调节在感染后肠功能紊乱发病中的作用。方法将60只雄性Wistar大鼠随机分为对照组和实验组,用福氏志贺氏痢疾杆菌灌胃制造感染后肠功能紊乱大鼠模型;于感染后第23天,将两组大鼠于水合氯醛麻醉下处死,留取腹主动脉血清;应用酶联免疫吸附试验(ELISA)对感染后肠功能紊乱大鼠外周血Th1型细胞因子IL-1β和Th2型细胞因子IL-4的含量进行检测分析。结果与对照组比较,实验组大鼠外周血Th1型细胞因子IL-1β表达有升高趋势,但差异无统计学意义(P>0.05);Th2型细胞因子IL-4表达较对照组显著降低(P<0.05);Th1/Th2的比值较对照组显著升高(P<0.05)。结论感染后肠功能紊乱大鼠外周血中细胞因子产生了失衡,呈Th1优势反应,提示其存在轻微炎性反应和免疫调节异常。
Objective To detect and analyze Th1-type cytokine IL-1β and Th2-type cytokine IL-4 levels in the peripheral blood of post-infective intestinal dysfunction of rats,and to explore the role of inflammatory factors and immune regulation in the pathogenesis of post-infective intestinal dysfunction.Methods The 60 male Wistar rats were randomly divided into control group and experimental group,the post-infective intestinal dysfunction of rat model was induced by gavage with Shigella flexneri.In the 23th day after infection,the two groups of rats were killed by chloral hydrate anesthesia,Serum specimens were removed from abdominal aorta,and the content of blood cytokines IL-1β,IL-4 was measured by enzyme-linked immunosorbent assay（ELISA）.Results Compared with control group,the expression of peripheral blood Th1-type cytokines IL-1β of experimental group rats tended to increase,but the difference was not significant（P0.05）,and the expression of blood Th2-type cytokines IL-4 was reduced significantly （P 0.05）,and the ratio of Th1/Th2 was increased significantly（P0.05）.Conclusion The peripheral blood cytokines of post-infective intestinal dysfunction of rats had become imbalance with Th1 dominant response,indicating there was a slight inflammation and immune regulation abnormalities.
出处
《中国临床保健杂志》
CAS
2010年第2期183-185,共3页
Chinese Journal of Clinical Healthcare
