摘要
以蝴蝶兰花梗为初次诱导外植体,对蝴蝶兰的离体繁殖途径进行研究,建立蝴蝶兰植株再生系统。结果表明:初代培养基:MS+6-BA 5 mg/L+NAA 0.3 mg/L+蔗糖30 g/L+琼脂6 g/L的萌芽数最多;增殖培养基:MS+6-BA 7 mg/L+NAA 1.0 mg/L+蔗糖30 g/L+琼脂6 g/L+椰汁200 mL/L叶片数和增殖系数最高;生根诱导培养基:改良1/2 KC+IBA 1 mg/L+NAA 0.5 mL/L生根效果最好。
The flower-stems of the plant were used as explants primarily induced to study the propagation pathway of Phalaenopsis Hybrid cultured in vitro, and the regeneration systems of the plant were constructed. The result indicated that the most numbers of the sprouts were observed by using MS medium supplemented with 6-BA 5 mg/L, NAA 0. 3 mg/L, Sugar 30 g/L and Agar 6 g/L as primary culture medium;the optimal propagation medium for the highest numbers of leaf and propagation coefficient was MS medium supplemented with 6-BA 7 mg/L, NAA 1.0 mg/L, Sugar30 g/ L, Agar 6 g/L and Coconut juice 200 mL/L;the optimal root medium was half of the modified KC medium supplemented with IBA 1 mg/L and NAA 0. 5 mL/L.
出处
《北方园艺》
CAS
北大核心
2010年第8期122-124,共3页
Northern Horticulture
关键词
蝴蝶兰
花梗
外源激素
组织培养
Phalaenopsis Hybrid
flower-stem
growth substance
tissue culture
