摘要
以红颜草莓(Fragaria×ananassa Duch‘Benihoppe’组培苗为材料进行叶片离体再生研究,建立)了一个高效的叶片再生体系。结果表明:暗培养10天是叶片诱导愈伤组织最适合的暗培养时间。适宜的叶片诱导愈伤培养基为MS+TDZ2.0mg/L+NAA0.1mg/L,出愈率95.0%;适宜的愈伤诱导分化培养基为MS+TDZ1.0mg/L+NAA0.1mg/L,分化率90.0%;最适宜的生根培养基为1/2MS,生根率为100%。
An efficient method for plant regeneration from leaf explant o‘f Benihoppe’strawberry （Fragaria × ananassa Duch） was established. The optimal callus inducing medium for Benihoppe’strawberry leaves was MS＋TDZ 2.0 mg/L ＋NAA 0.1 mg/L, and the callus induction rate was 95.0%.The optimal differentiation medium was MS＋TDZ1.0 mg/L＋NAA0.1 mg/L, and the differentiation rate was 90.0%. The optimal choice which could induce callus of the leaves was darkness treatment for 10 days.
出处
《中国农学通报》
CSCD
北大核心
2010年第10期246-249,共4页
Chinese Agricultural Science Bulletin
基金
北京市教委科技发展计划项目"草莓抗褐化新品系选育与脱毒快繁"(KM200710020012)
关键词
红颜草莓
组织培养
叶片再生
暗培养
Benihoppe Strawberry
tissue culture
leaf regeneration
dark treatment
