摘要
目的探讨miR-181a和miR-181b对胶质瘤细胞增殖和侵袭等影响。方法通过实时荧光定量PCR检测miR-181a和miR-181b在胶质瘤组织和细胞株中的表达情况。我们合成miR-181a和miR-181b寡聚核苷酸及构建pre—miR-181a和pre—miR-181b表达载体，转染胶质瘤细胞，通过MTF、Transwell实验、流式检测和软琼脂实验，观察上调miR-181a和miR-181b表达后对胶质瘤细胞增殖、侵袭、转化能力和细胞凋亡的影响。结果miR-181a和miR-181b在胶质瘤组织和细胞株较正常脑组织均呈过低表达；miR-181a表达水平与胶质瘤等级呈负相关。上调miR-181a和miR-181b表达能有效抑制胶质瘤细胞生长，降低其转化和侵袭能力，诱导凋亡。结论胶质瘤中异常低表达的miR-181a和miR-181b可能发挥着肿瘤抑制因子作用。
Objective To study the effects of miR - 181a and miR - 181b overexpression on proliferation, transformation, apoptosis, and invasion of glioma cells. Methods The relative expression of miR - 181a and miR - 181b in human glioma tissue samples and glioma cell lines were performed by real - time quantification RT -PCR. We chemically synthesized miR -181a and miR- 181b oligonucleotides and constructed expression vectors for pre - miR - 181a and pre - miR - 181b. The effects of the proliferation, transformation, invasion, and apoptosis of miR -181a and miR- 181b on transfected cells were evaluated via MTT assay, soft agar colony assay, transwell assay and flow cytometric method. Results MiR - 181a and miR - 181b were strongly down - regulated in all glioma samples and glioma cell lines, and miR - 181a expression was negatively correlated with tumor grades. Overexpression of miR - 181a and miR - 181b could effectively lead to glioma cells growth suppression, transformation inhibition, apoptosis increase, and invasion reduction. Conclusion The aberrantly down - regulated miR - 181a and miR - 181b may act as tumor suppressors in human glioma.
出处
《中华神经外科杂志》
CSCD
北大核心
2010年第4期365-368,共4页
Chinese Journal of Neurosurgery
基金
基金项目：国家自然科学基金项目资助（30672165,30772231,30872657）：江苏省医学重点人才项目（RC2007061）
