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PAK5对大肠癌细胞黏附及迁移的影响 被引量：1

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摘要 目的研究PAK5对大肠癌细胞黏附以及迁移的影响。方法采用基因转染上调PAK5在LST细胞中的表达水平,以及RNA干扰下调PAK5在SW480细胞中的表达水平,采用细胞黏附以及迁徙实验检测PAK5的黏附、迁徙能力;结果采用基因转染上调PAK5在LST细胞株中的表达水平后,细胞-基质黏附能力减弱,迁徙能力增强;相反,采用RNA干扰敲低PAK5在SW480细胞株中的表达水平后,细胞-基质黏附能力增强,迁徙能力减弱。结论PAK5通过减弱细胞-基质黏附,增强大肠癌细胞的迁徙能力。
作者 龚伟 姜泊 张宏权
机构地区 南方医科大学南方医院消化内科
出处 《广东医学》 CAS CSCD 北大核心 2010年第7期820-822,共3页 Guangdong Medical Journal
基金 南方医院院长基金资助项目(编号:20070001)
关键词 PAK5 大肠癌 细胞黏附与迁移
分类号 R735.34 [医药卫生—肿瘤]
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参考文献8

  • 1DAN C,NATH N,LIBERTO M,et al.PAK5,a new brain-specific kinase,promotes neurite outgrowth in N1E-115 cells[J].Mol Cell Biol,2002,22(2):567-577.
  • 2BRYAN B,KUMAR V,STAFFORD L J,et al.GEFT,a Rho family guanine nucleotide exchange factor,regulates neurite outgrowth and dendritic spine formation[J].J Biol Chem,2004,297(44):45824-45832.
  • 3MATENIA D,GRIESSHABER B,LI X Y.PAK5 kinase is an inhibitor of MARK/Par-1,which leads to stable microtubules and dynamic actin[J].Mol Biol Cell,2005,16(9):4410-4422.
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同被引文献10

  • 1Dan C, Nath N, Liberto M, et al. PAK5, a new brain-specific kinase, promotes neurite outgrowth in N1E-115 cells. Mol cell Biol, 2002, 22(2):567-577.
  • 2Bryan B, Kumar V, Stafford LJ, et al. GEFT, a Rho family guanine nucleotide exchange factor, regulates neurite outgrowth and dendrit- ic spine formation. J Biol Chem, 2004,297(44):45824-45832.
  • 3Matenia D, Griesshaber B, Li XY, et al. PAK5kinase is an inhibitor of MARK/Par-1, which leads to stable microtubules and dynamic actin. Mol Biol Cell, 2005,16(9):4410-4422.
  • 4Cotteret AI, Jaffer ZM, Beeser A, et al. p21-activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phospho- rylating BAD. Mol Cell Biol, 2003,23(16):5526-5539.
  • 5Cotteret S, Chernoff J. Nucleocytoplasmic shuttling of pak5 regu- lates its antiapoptotic properties. Mol Cell Biol,2006,26 (8):3215- 3230.
  • 6Wells CM, Jones GE. The emerging importance of group II PAKs. Biochem J, 2010,425(3):465-473.
  • 7Wu X, Frost JA. Multiple Rho proteins regulate the subcellular tar- geting of PAK5. Biochem Biophys Res Commun, 2006,351(2):328- 335.
  • 8Bokoch GM. Biology of the p21-activated kinases.Ann Rev Biochem, 2003,72:743-781.
  • 9Bagheri-Yarmand R, Mandal M, Taluker AH, et al. Etk/Bmx tyro- sine kinase activates Pak1 and regulates tumorigenicity of breast cancer cells. J Biol Chem, 2001;276(31):29403-29409.
  • 10Carter JH, Douglass LE, Deddens JA, et al. Pak-1 Expression In- creases with Progression of Colorectal Carcinomas to Metastasis. Clinical Cancer Research, 2004,10(10):3448-3456.

引证文献1

二级引证文献2

广东医学
2010年 第7期

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