摘要
目的探讨二烯丙基二硫(DADS)对人胃癌SGC7901细胞增殖及周期分布的影响。方法体外培养SGC7901细胞,采用MTT比色实验、细胞计数法和流式细胞术分析DADS对SGC7901细胞增殖的抑制作用与对细胞周期分布的影响。结果MTT法显示,不同浓度DADS(50、100、150、200、250μmol/L)处理SGC7901细胞96 h后,生长抑制率分别为21.07%、49.96%、59.73%、67.64%、70.49%,DADS抑制作用随浓度增加逐渐增强(P<0.05)。细胞计数法表明:常规培养的SGC7901细胞群体倍增时间分别为24.35 h,当DADS浓度由50μmol/L增加到200μmol/L时,其细胞群体倍增时间由27.28 h增加到71.98 h(P<0.05)。流式细胞仪分析发现,50、100和200μmol/L DADS呈浓度依赖性将SGC7901细胞阻滞在G2/M期。结论DADS具有抑制人胃癌SGC7901细胞增殖的作用,且其抑制增殖作用与G2/M期阻滞有关。
Objective To investigate the effect of diallyl disulfide （DADS） in the proliferation and cell cycle of human Gastric cancer cell line SGC790. Methods The experiment was divided into six groups, untreated group and treatment group with 50, 100, 150, 200, 250 μmol/L DADS. SGC7901 cells cultured in vitro, the growth inhibition of SGC7901 cell line was measured by MTT assay. Phase distribution of cell cycle was analyzed by flow cytometry. Results SGC7901 cells were treated with 50, 100, 150,200,250 μmol/L DADS for 96 h, the proliferation inhibition rates were 21.07%, 49.96%, 59.73%, 67.64%, 70.49% respectively contrast with control（ P 〈0.05 ）, which exhibited a dose - dependent model by MTT test. Cell counting showed that SGC7901 cells population doubling time was only 24.35 h ; when treated with DADS from 50 μmol/L to 200 μmol/L, the time increased from 27.28 h to 71.89 h （ P 〈 0.05 ）. After treated with 50, 100, 150 μmaol/L DADS for 24 h, 48 h, flow cytometry analysis revealed that SGC7901 cells were arrested in the G2/M phase in a dose - dependent model （ P 〈 0.05）. Conclusion DADS significantly inhibited the proliferation of SGC7901 cells which may be induced by G2/M arrest of DADS.
出处
《南华大学学报（医学版）》
2010年第2期197-199,218,共4页
Journal of Nanhua University(Medical Edition)
基金
国家自然科学基金(编号30600285)
南华大学博士科研启动基金资助项目(2007XQD26)
