摘要
[目的]探讨不同碳源和氮源对橡胶炭疽菌菌丝生长和孢子萌发的影响。[方法]采用查理固体培养基和孢子悬浮液培养,通过对菌丝生长和孢子萌发的测定试验,研究10种碳源和9种氮源对橡胶炭疽菌菌丝生长和孢子萌发的影响。[结果]菌丝生长试验表明,乳糖、D-果糖、葡萄糖、甘油、D-木糖5种碳源培养基的菌丝体生长量较好,生长量均≥7.87mm/d,L-山梨糖碳源培养基则效果较差;硝酸钠、甘氨酸、L-赖氨酸、L-组氨酸4种氮源培养基的菌丝体生长量较好,其生长量≥8.00mm/d,硫酸铵、硝酸铵、氯化铵、L-半胱氨酸氮源培养基则效果较差。孢子萌发试验说明,橡胶炭疽菌能较好地利用各种碳源和氮源,孢子萌发率最低的是在甘油碳源溶液中。[结论]对菌丝体生长和孢子萌发最有利的碳源是葡萄糖,对菌丝生长和孢子萌发较好的氮源有硝酸钠和L-组氨酸。
[Objective] The study aimed to explore the effects of different carbon source and nitrogen sources on the mycelium growth and spore germination of Colletrichum.[Method] The Charlie solid medium and the conidial suspension culture were used to study the the effects of 10 carbon sources and 9 nitrogen sources on the mycelium growth and spore germination of Colletrichum through the determination test on the mycelium growth and spore germination.[Result] The mycelium growth test showed that the mycelium growth in 5 kind of carbon source medium with lactose,D-fructose,glucose,glycerin,D-xylose were better,with their growth amount all being ≥7.87 mm/d and the carbon sources medium with L-sorbin got the poor effect. The mycelium growth in 4 kind of nitrogen source medium with sodium nitrate,glycin,L-lysine and L-histidine were better,with their growth amount all being ≥ 8.00 mm/d. The nitrogen source medium with ammonium sulfate,ammonium nitrate,ammonium chloride and L-cystine got the poor effects. The spore germination test showed that Colletrichum could better use the various carbon sources and nitrogen sources and the glycerin carbon source solution got the lowest the germination rate of Colletrichum spore.[Conclusion] The optimum carbon sources to the mycelial growth and spore germination was glucose and the optimum nitrogen sources to the one were sodium nitrate and L-histidine.
出处
《安徽农业科学》
CAS
北大核心
2010年第11期5711-5715,5793,共6页
Journal of Anhui Agricultural Sciences
基金
中央级公益性科研院所基本科研业务专项资助项目(2009hzs1J015)
海南省重点科技项目(090141)
国家科技支撑计划项目(2007BAD48B04)
关键词
橡胶炭疽菌
菌丝生长
孢子萌发
炭源
氮源
Colletrichum
Carbon sources
Nitrogen sources
Mycelium growth
Spore germination
