摘要
[目的]探讨硫酸软骨素酶在治疗急性脊髓损伤中的作用,评价该方法的疗效。[方法]40只Wistar大鼠,利用Impactor model II型脊髓打击器制造T10节段急性脊髓损伤模型,随机分成两组:对照组(A组)和实验组(B组)。实验组应用硫酸软骨素酶ABC治疗,对照组给予生理盐水。采用脊髓运动功能评分(BBB法)、神经电生理(SEP&MEP)检查和BDA神经顺行示踪以及NF-200免疫组化染色等方法评价各组疗效。统计学处理采用t检验。[结果]手术4周后BBB行为功能评分以及神经电生理检查实验组较对照组功能恢复明显,差异有统计学意义(P<0.05)。HE染色显示实验组空洞和瘢痕较对照组少;BDA示踪显示实验组较空白对照组有较多的神经纤维穿过缺损部位。NF-200免疫组化染色阳性面积比较两组之间差异有统计学意义(P<0.05)。[结论]硫酸软骨素酶ABC能降解胶质瘢痕中主要的抑制分子CSPGs,改善脊髓损伤后局部轴突再生的抑制性微环境,从而能显著促进脊髓损伤后轴突再生。
[Objective]To investigate the effect of chondroitinase ABC（ChABC） after spinal cord injury（SCI） in adult rats,and to evaluate the significance of treatment. [Methods]Forty adult Wistar rats were divided into two groups： the control group（group A）,the chondroitinase ABC group（group B）.The thoracic T10 spinal cord injury model was made by impactor model II manual.After SCI,group B animals received ChABC treatment,while group A animals only received blank saline.The locomotor functions（BBB score） and the neuroelectrophysiological changes（SEPMEP） of the rats were evaluated.Twelve weeks after SCI,biotinylated dextran amine（BDA） was injected into the cerebral cortex to trace the corticospinal tracts（CST）.Then two weeks after the tracing,the animals were killed and the spinal cord frozen sections were made.The tissue sections were processed with Cy3 fluorescence stain,HE stain and anti NF-200 immuohistological stain.The section images were analyzed by Image Pro Plus image analyzing software.All the statistics were performed by SPSS 13.0 software.[Results]At 4 weeks after SCI,group B had obvious higher BBB scores than control group（P0.05）,indicating better locomotor recovery.In electromyographic examination（SEPMEP）,similar results were got.Group B animals gained better recovery than those in group A（P0.05）.In histological observation,HE stain showed more and bigger cavities in control group than other groups,and even more glia scars.In BDA tracing slides,the corticospinal tract axons could hardly cross the injury sites in control group,while some axons could cross the injury sites in ChABC group.The anti-NF-200 stain areas were different between the two groups,with statistical significance（P0.05）.[Conclusion]Chondroitinase ABC can degrade the chondroitin sulfate proteoglycans which inhibit axon regeneration in glia scar,and can inprove the inhibitory environment of injury site,thus promoting axon regeneration and functional recovery.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2010年第7期573-578,共6页
Orthopedic Journal of China
基金
国家自然科学基金资助项目(30872603)
天津市应用基础和前沿技术支持计划资助项目(07JCBJC10200)
教育部新世纪人才支持计划资助项目(NCET-06-0251)
关键词
脊髓损伤
再生
软骨素酶
spinal cord injury
regeneration
chondroitinase
