摘要
目的 探讨CD123在淋巴细胞白血病中的表达特点及意义.方法 应用多参数流式细胞术检测139例淋巴细胞白血病患者CD123的表达.以10名正常人骨髓淋巴细胞作对照.对105例急性B淋巴细胞白血病（B-ALL）患者行细胞遗传学分析.对97例B-ALL患者进行白血病微量残留病（MRD）分析.结果 ①10名正常人骨髓中B淋巴干/祖细胞、成熟B淋巴细胞及T淋巴细胞均不表达CD123.139例淋巴细胞白血病患者中,5例T-ALL和23例慢性B淋巴细胞白血病（B-CLL）患者CD123均阴性.111例B-ALL患者中106例CD123阳性（阳性率95.49%）,其中包括12例早期前B-ALL、57例普通B-ALL、37例前B-ALL;5例成熟B-ALL患者CD123阴性.②在B-ALL患者中,CD123表达水平与p-Akt表达水平呈正相关,超二倍体患者组CD123表达水平高于非超二倍体患者组.③MRD阳性与阴性组患者比较,12个月内复发率分别为63.04%和21.56%,差异有统计学意义（P〈0.01）.MRD阴性组无病生存率[（48.23±1.82）%]高于MRD阳性组[（36.06±2.62）%].CD123在复发B-ALL患者中表达稳定性高.结论 CD123在B-ALL患者中普遍中度至强度表达,复发时表达稳定性高,是MRD分析较好的标志之一.
Objective To investigate the expression of CD 123 and its significance in lymphocytic leukemia.Methods CD123 expression in 139 lymphocytic leukemia patients and in lymphocytes from 10 normal bone marrows（ BM） was analyzed by multi-parameter flow cytometry.Cytogenetic and minimal residual disease （MRD） analysis were performed in acute B-lymphocytic leukemia （B-ALL） patients.Results CD123 expression was absent in B lymphoid lineage stem-progenitor cells, mature B and T lymphocytes from 10 normal BM.Among 139 lymphocytic leukemia patients, CD123 was negative in 5 T-ALL and 23 B-CLL patients.However, among 111 B-ALL patients, CD123 was expressed in 106 （12 pro B-ALL, 57 common B-ALL and 37 Pre B-ALL） （95.49% ） but not in 5 mature B-ALL patients.There was a positive correlation between CD123 and p-Akt expression, and CD123 expression was much higher in hyperdiploid than in non-hyperdiploid B-ALL patients.A statistically significant difference in relapse rate within 12 months （MRD positive group： 63.04% vs MRD negative group 21.56% ） and in disease free survival （ DFS） time was found be-ween patients with MRD[（36.06 ±2.62）%] or not [（48.23 ± 1.82）%] （P〈0.01）.Moreover, stable CD123 expression could be observed in B-ALL patients in relapse.Conclusions CD123 was predominantly expressed in B-ALL patients and remained in patients in relapsec, indicating that it may be an useful MRD marker in B-ALL patients.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2010年第4期244-248,共5页
Chinese Journal of Hematology
