摘要
目的比较不同肝癌细胞株对5-氮杂-2′-脱氧胞苷(5-aza-dC)的敏感性,探讨肝癌细胞对5-aza—dC的敏感性是否与细胞总DNA甲基化水平有关。方法用不同剂量(0.5、5.0、10.0μmol/L)的5-aza—dC处理肝癌细胞株(HepG2、QGY7701和HepG2.2.15细胞)及正常肝细胞株L02,比较不同浓度处理前后的细胞增殖抑制率,比较10μmol/L 5-aza-dC处理前后的Caspase-3活性及细胞DNA片段化水平(5-溴脱氧尿嘧啶核苷掺入率),比较不同细胞总DNA甲基化水平。组间检测结果比较采用t检验。结果5-aza—dC对HepG2、QGY7701、HepG2.2.15、L02细胞的半数抑制浓度分别为0.5、0.5、4.5、11.4μmol/L,与HepG2细胞和QGY7701细胞相比,HepG2.2.15细胞和L02细胞对5aza—dC不敏感。HepG2和QGY7701细胞中Caspase-3的活性升高较L02和HepG2.2.15细胞明显(P值均〈0.05),QGY7701细胞中5-溴脱氧尿嘧啶核苷掺入率升高较L02细胞明显(P〈0.05)。L02、HepG2、QGY7701和HepG2.2.15细胞的DNA总甲基化水平分别为11.7%±0.9%、10.9%±1.3%、11.7%±1.7%和12.2%±1.0%,差异无统计学意义(P值均〉0.05)。结论细胞对5-aza-dC的敏感性与细胞总DNA甲基化水平无关。
Objective To compare the sensitivity of different hepatocelullar carcinoma (HCC) cell lines (HepG2, QGY7701, HepG2.2.15) and the normal liver cell line L02 to 5-aza-dC, an DNA methyltransferase inhibitor, and to explore the relationship between global DNA methylation level and the sensitivity to 5-aza- dC. Methods HepG2, QGY7701, HepG2.2.15 and L02 cells were treated with 5-aza-dC at different concentration, cell proliferation was measured by MTT method, cell apoptosis was detected by measuring caspase 3 activity and cellular DNA fragmentation ELISA. Results Compared to HepG2 and QGY7701 cells, HepG2.2.15 were less sensitive to the treatment of 5-aza-dC; the normal liver cell line L02 was less sensitive to 5-aza-dC than the HCC cell lines. Conclusions The sensitivity to 5-aza-dC of HCC cell lines and normal liver cells is not correlated with the global DNA methylation level.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2010年第4期284-287,共4页
Chinese Journal of Hepatology
基金
重庆市自然科学基金(2007BB5308)