摘要
目的:探讨中药大黄素对乳腺癌MCF-7/Adr细胞株多药耐药的逆转作用,及其对细胞中ERCC1蛋白表达的影响,阐明大黄素逆转细胞耐药与ERCC1的关系。方法:采用MTT比色法进行药敏试验,蛋白质印迹法检测ERCC1蛋白的表达。结果:MCF-7/Adr对多柔比星和顺铂的耐药倍数分别为21和11倍,在加入10μg/mL大黄素后,耐药逆转倍数分别为2.86和1.79。10和20μg/mL大黄素处理MCF-7/Adr后第2、4、6、10天,ERCC1蛋白的表达呈逐渐下降趋势,在20μg/mL浓度下降低相对明显。结论:大黄素具有逆转MCF-7/Adr细胞多药耐药的作用,同时还下调了ERCC1的表达水平,并显示了一定的时间依赖性。
OBJECTIVE:To investigate the reversal effect of emodin,a traditional Chinese medicine,on multi-drug resistant breast cancer cell line MCF-7/Adr,and explore its influence on the expression of ERCC1. METHODS:MTT assay was used for drug sensitivity tests,and Western Blot was used for ERCC1 protein. RESULTS:The resistance multiples of adriamycin and cisplatin were 21 and 11 in MCF-7/Adr. The reversal multiples of drug resistance were 2.86 and 1.79,respectively,after emodin(10 μg/mL) added. MCF-7/Adr cells were treated with two concentrations (10 and 20 μg/mL) of emodin,after 2,4,6 and 10 days,the trend of ERCC1 expression was gradually downward,It was more obvious comparatively at the concentration of 20 μg/mL. CONCLUSION:Emodin can reverse the multi-drug resistance on MCF-7/Adr cells,and down-regulate the expression of ERCC1 protein in a time-dependent manner.
出处
《中华肿瘤防治杂志》
CAS
2010年第1期27-29,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
深圳市科技和信息局科技计划重点项目(200601019)
深圳市卫生局重点课题项目(200628)