摘要
应用正交设计法考察不同培养基和4种植物生长物质对诱导金线兰愈伤组织生长的作用,研究了不同培养基对金线兰愈伤组织诱导的影响。结果表明,6-BA对诱导金线兰愈伤组织生长效果显著,2,4-D、NAA和KT效果不显著;金线兰愈伤组织诱导的最佳培养基为MS+2,4-D 3.0 mg/L+6-BA 1.0 mg/L+NAA 0.5 mg/L+KT 0.25 mg/L。
Culture medium and 4 plant growth substances were studied for their function to callus growth of A. roxburghii by orthogonal design. Results showed that 6-BA had significant effect on induced callus growth, while 2,4-D, NAA, and KT had not. The optimal culture medium for callus induction of A. roxburghii, was MS＋2,4 D 3.0mg/Lq＋BA 1.0mg/Lq-NAA 0.5 mg/L＋KT 0.25mg/L.
出处
《贵州农业科学》
CAS
北大核心
2010年第4期33-35,共3页
Guizhou Agricultural Sciences
基金
贵州省科学技术基金资助项目"信号物质对金线兰逆境蛋白产生的调控作用研究"[黔科合J字(2008)2097]
关键词
金线兰
诱导
愈伤组织
Anoectochilus roxburghii
induction
callus
