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Anaerobic benzene biodegradation by a pure bacterial culture of Bacillus cereus under nitrate reducing conditions 被引量:6

Anaerobic benzene biodegradation by a pure bacterial culture of Bacillus cereus under nitrate reducing conditions
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摘要 A pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil.The analysis of the 16S rDNA gene sequence,morphological and physiological characteristics showed that the isolated strain was a member of genus Bacillus cereus.The biodegradation performance of benzene by B.cereus was evaluated,and the results showed that benzene could be efficiently biodegraded when the initial benzene concentration was below 150 mg/L.The metabolites of anaerobic nitrate-dependent benzene oxidation by strain B.cereus were identified as phenol and benzoate.The results of substrate interaction between binary combinations for benzene,phenol and benzoate showed that the simultaneous presence of benzene stimulated the degradation of benzoate,whereas the addition of benzene inhibited the degradation of phenol.Benzene degradation by B.cereus was enhanced by the addition of phenol and benzoate,the enhanced effects were more pronounced at higher concentration.To our knowledge,this is the first report that the isolated bacterial culture of B.cereus can efficiently degraded benzene under nitrate reducing conditions. A pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil.The analysis of the 16S rDNA gene sequence,morphological and physiological characteristics showed that the isolated strain was a member of genus Bacillus cereus.The biodegradation performance of benzene by B.cereus was evaluated,and the results showed that benzene could be efficiently biodegraded when the initial benzene concentration was below 150 mg/L.The metabolites of anaerobic nitrate-dependent benzene oxidation by strain B.cereus were identified as phenol and benzoate.The results of substrate interaction between binary combinations for benzene,phenol and benzoate showed that the simultaneous presence of benzene stimulated the degradation of benzoate,whereas the addition of benzene inhibited the degradation of phenol.Benzene degradation by B.cereus was enhanced by the addition of phenol and benzoate,the enhanced effects were more pronounced at higher concentration.To our knowledge,this is the first report that the isolated bacterial culture of B.cereus can efficiently degraded benzene under nitrate reducing conditions.
出处 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2010年第5期709-715,共7页 环境科学学报(英文版)
基金 supported by the National Natural Science Foundation of China (No. 40873076,40773055) the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry (No.2008890)
关键词 Bacillus cereus nitrate reduction anaerobic biodegradation substrate interaction PHENOL BENZOATE Bacillus cereus nitrate reduction anaerobic biodegradation substrate interaction phenol benzoate
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  • 1HU Zhi-feng,DOU Jun-feng,LIU Xiang,ZHENG Xi-lai,DENG Dong.Anaerobic biodegradation of benzene series compounds by mixed cultures based on optional electronic acceptors[J].Journal of Environmental Sciences,2007,19(9):1049-1054. 被引量:10
  • 2Alvarez P J J,Vogel T M,1991.Substrate interactions of benzene,toluene,and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries[J].Appl Environ Microbiol.57:2981-2985.
  • 3Beller H R,Spormann A M,1998.Analysis of the novel benzylsuccinate synthase reaction for anaerobic toluene activation based on structural studies of the product[J].J Bacteriol,180:5454-5457.
  • 4Burland S M,Edwards E A,1999.Anaerobic benzene biodegradation linked to nitrate reduction[J].Appl Environ Microbiol.65:529-533.
  • 5Chiang C,Salanitro J,Chai E et al.,1989.Aerobic biodegradation of bcnzene,toluene,and xylene in a sandy aquifer:data analysis and computer modeling[J].Ground Water,27(6):823-834.
  • 6Coates J D,Anderson R T,Lovley D R,1996.Oxidation of polycyclic aromatic hydrocarbons under sulfate-reducing conditions[J].Appl Environ Microbiol,62:1099-1101.
  • 7Coates J D,CHakraborty R,Lack J G et al.,2001.Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas[J].Nature,411:1039-1043.
  • 8Coates J D,Chakraborty R,Mclnerney M J,2002.Anaerobic benzene biodegradation-a new afea[J].Res Microbiol,153:621-628.
  • 9Heider J,Boll M,Breese K et al.,1998.Differential induction of enzymes involved in anaerobic metabolism of aromatic compounds in the denitrifying bacterium Thauera aromatica[J].Arch Microbiol,170:120-131.
  • 10Langenhoff A A M,Zehnder A J B,Schraa G,1996.Behaviour of toluene,benzene and naphthalene under anaerobic conditions in sediment columns[J].Biodegradation,7:267-274.

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