摘要
用胚胎固定脱膜方法对黑尾近红鲌胚胎发育进行连续观察,用整胚原位杂交技术对原始生殖细胞的起源和迁移进行研究。试验结果表明,黑尾近红鲌受精卵为粘性,淡黄色,圆形,直径(0.94±0.05)mm。在水温24.5℃的孵育条件下,受精卵经25 min开始胚胎发育,45 min进入2细胞期,5 h15 min进入囊胚期,7 h 25 min进入原肠期,10 h 15 min进入神经胚期;50 h,50%以上的仔鱼孵出。刚出膜的仔鱼长约(4.10±0.02)mm。黑尾近红鲌原生殖细胞最早发现于原肠期;在原肠中期和神经胚期,其位于胚盾靠近卵黄囊的内胚层;在胚层分化期,原生殖细胞迁移到脏壁中胚层;在孵化期,原生殖细胞迁移到体两侧的生殖嵴中,与生殖嵴共同组成胚胎的未分化性腺。
Embryo fixation technique was used to observe the embryonic development and to study the origin and migration of primordial germ cells（PGC）in culter Ancherythroculter nigrocauda. It was found that the glutinous yellow oval fertilized eggs with a diameter of （0.94±0.05） mm developed into two-cell stage about 45 rain after fertilization（AF）, blastula stage 5 h 15 min AF, gastrula stage 7 h 25 rain AF, neural stage 10 h 15 rain AF, and 50o/60 of newly-hatched larvae 50 h AF at water temperature of around （24.5℃）. The newly-hatched fry had body length （4. 10±0.02） mm. The PGCs were first observed in gastrula stage, at the blastoderm margin and subsequently remained in the hypoblast of the embryonic shield region during mid-gastrula and neural stage. Later they migrated to the splanchnic mesoderm during the stage of germ layer differentiation. Finally, they translocated to the genital ridges where they developed into the undifferentiated gonad.
出处
《水产科学》
CAS
北大核心
2010年第5期265-269,共5页
Fisheries Science
基金
郑州市科技攻关项目(No.083SGYG25121-8)
关键词
黑尾近红鲌
胚胎发育
原生殖细胞
迁移
Ancherythroculter nigrocauda
embryonic development
PGC
migration
