摘要
目的 探讨肠内营养联合不同剂量人参皂甙Rb1对70％中段小肠切除一吻合术模拟的术后疲劳综合征（POFS）大鼠模型的疗效及作用机制。方法采用随机数字表法将72只雄性Sprague-Dawley远交群大鼠分为对照组（C组）、模型组（M组）、肠内营养组（EN组）、EN＋高剂量Rb1组（EHP组）、EN＋中剂量Rb1组（EMP组）、EN＋低剂量Rb1组（ELP组），每组12只。术后检测体重变化，采用Morris水迷宫实验和改良悬尾实验评估大鼠的学习与记忆保持能力、体力、耐力及精神状况；采用ELBA法测定血清转铁蛋白、前白蛋白、纤维连接蛋白及白细胞介素2含量，溴甲酚绿比色法测定血清白蛋白含量，采用流式细胞术测定CD4＋、CD8＋细胞比例。结果除M组外，各组体重交替上升，各组间的差异无统计学意义（P〉0．05）。EN与Rb1联合营养支持组的潜伏期显著短于M组（P〈0．05），EHP组、EMP组的准确穿越平台次数显著高于M组（P〈0．01）。EHP组和EMP组的挣扎域上面积均显著大于M组（P〈0．05）；EHP组、EMP组和ELP组的休息累积不动时间均显著短于M组（P均〈0．05）。EN与Rb1联介营养支持组的血清转铁蛋白、前白蛋向、纤维连接蛋白含量均显著高于M组（P〈0．05）。3个含Rb1营养支持绀的CD4＋T细胞比例和自细胞介素2水平均显著高于M组（P〈0．05），而CD8＋T细胞比例与M组的差异尤统计学意义（P〉0．05）。结论EN与Rb1联合营养支持能明显改善术后疲劳情况，与Rb1剂量有关；其可能机制为增强术后营养、遏制高代谢、增加免疫力。
Objective To explore the therapeutical effects of enteral nutrition （EN） combined with panaxoside Rh1 on mouse modles of postoperative fatigue syndrome. Methods Totally, 72 male Sprague-Dawley mice were randomly divided into control group, model group, EN group, EN associated with high/middle/low dose panaxoside Rb1 groups （EHP group, EMP group, and ELP group, n= 12 in each group）. Changes in body weight were measured before and after interventions. Learning and memory playback abilities, physical strength, and vim state were evaluate by Morris Water Maze test and Improved Tail Suspension test. Serum transferrin, prealbumin, fibroneetin, and interleukin-2 levels were measured with ELISA. Serum albumin level was assayed with Bromeresol Green eolorimetrie technique. CD4＋ and CD8＋ proportions were assayed by flow cytometry. Results The body weight grew alternately in each group without significant differences （ P 〉 0. 05 ） except for model group. The latency period was significantly shorter in EN combined with panaxoside Rb1 group than that in model group （P 〈 0.05 ） , and the frequencies of crossing platform in EHP group and EMP group were significantly higher than those in model group （P 〈0. 01 ）. The areas of struggling above domain in EHP group and EMP group were signifi cantly larger than those in model group （P 〈0. 05 ） , and the aecumulated static time of rest in EHP group, EMP group, and ELP group was significantly shorter than that in model group （P 〈 0. 05）. Serum transferrin, prealburain, fibronectln levels in EN combined with panaxoside Rbl group were significantly higher than those in model group （ all P 〈 0. 05 ）. The CD4 ＋ T proportion and interleukin-2 level in EHP group, EMP group, and ELP group were significantly higher than those in model group （ both P 〈 0.05 ） ; however, CD8 ＋ T proportion was not significantly different between three panaxoside Rbl groups and model group （ P 〉 0. 05 ）. Conclusion EN combined with panaxoside Rbl can improve postoperative fatigue syndrome in a dose-dependent way, which may be explained by the fact that it can strengthen the postoperative nutrition, restrain hypermetabolism, and increase immunity.
出处
《中华临床营养杂志》
CAS
2010年第1期38-41,共4页
Chinese Journal of Clinical Nutrition
基金
基金项目：浙江省医学重点扶植学科（07-F03）和浙江省中药管理局中药科技计划项目（2006C096）
