摘要
目的:研究细胞外信号调节激酶(Erk1/2)和蛋白激酶B(PKB/Akt)在去势大鼠阴茎海绵体中的表达及活性,探讨其在去势大鼠勃起功能障碍(ED)发生中的作用机制。方法:20只8周龄雄性SD大鼠随机分成假手术组(A组)和去势组(B组),术后4周检测两组大鼠血清睾酮(T)水平,免疫组化及RT-PCR检测磷酸化Erk1/2和磷酸化PKB/Akt活性蛋白(累积光密度/面积,IA/Area)及Erk1/2、Akt mRNA(内参为GAPDH)在大鼠阴茎海绵体中的表达。结果:术后4周B组血清T水平[(1.339±0.642)nmol/L]较A组[(10.090±3.026)nmol/L]显著降低(P<0.05),Erk1/2和PKB/Akt在两组大鼠阴茎海绵体中均有表达,Erk1、Erk2的mRNA和磷酸化Erk1/2蛋白的相对表达量在B组(0.840±0.062、0.876±0.141、0.142±0.020)较A组(0.479±0.090、0.599±0.100、0.119±0.029)显著升高(P均<0.05);PKB/Akt mRNA和磷酸化PKB/Akt蛋白的相对表达量在B组(0.974±0.040、0.164±0.036)与A组(0.942±0.054、0.162±0.025)差异无显著性(P>0.05)。结论:Erk1/2及PKB/Akt在去势组及假手术组大鼠阴茎中均有表达。去势大鼠阴茎海绵体磷酸化Erk1/2表达增强与ED发生有关。
Objective：To study the expressions of Erk1/2 and PKB/Akt in the corpus cavernosum of castrated rats and investigate their action mechanism in the development of erectile dysfunction（ED） after castration.Methods：We randomly divided 20 eight-week-old SD rats into Groups A（sham-operation） and B（castration）,and,4 weeks after the operation,determined the level of serum testosterone（T） and the expressions of P-Erk1/2 and P-PKB/Akt proleins（integrated optical density/area,IA/area） and those of Erk1/2 and PKB/Akt mRNA（Marker;GAPDH） in the corpus cavernosum of the rats by immunohistochemical staining and RTPCR. Results：Four weeks after the operation,the serum T level was significantly decreased in Group B in comparison with A （[10.090±3.026]nmol/L versus[1.339±0.642]nmol/L,P0.05）.Erk1/2 and PKB/Akt expressed in the corpus cavernosum of both groups of rats.The expressions of Erk1 and Erk2 mRNA and P-Erk1/2 were significantly higher in Group B（0.840±0.062,0.876±0.141 and 0.142±0.020） than in A（0.479±0.090,0.599±0.100 and 0.119±0.029）（P0.05）. But no statistically significant differences were found in the expressions of PKB/Akt mRNA and P-PKB/Akt between Groups B （0.974±0.040 and 0.164±0.036） and A（0.942±0.054 and 0.162±0.025）（P0.05）.Conclusion：Erk1/2 and PKB/Akt expressed in the penile tissues of both castrated and sham-operation rats.The increased expression of P-Erk1/2 in the corpus cavemosum may be involved in the development of ED in castrated rats.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2010年第2期112-117,共6页
National Journal of Andrology
基金
四川省科技厅基金(060045)
四川省卫生厅基金(2006J13-146)
四川省教育厅研究基金(2005B017)~~
