摘要
目的探讨人脐血源基质细胞(human umbilical cord blood-derived stromal cells,hUCBDSCs)联合脐血单个核细胞(human umbilical cord blood-mononuclear cells,hUCB-MNCs)共移植促进放射损伤BALB/c小鼠造血重建与植入的效应。方法BALB/c近交系小鼠经60Coγ射线8.5Gy辐照后1d按照完全随机设计分为4组,经尾静脉分别输注生理盐水、hUCB-MNCs(1×107/只)、hUCB-MNCs(1×107/只)联合hUCBDSCs(1×106/只)及hUCB-MNCs(1×107/只)联合人骨髓基质细胞(hBMSCs,1×106/只)。检测移植后各组小鼠存活率、外周血象、骨髓病理变化、成纤维细胞集落形成单位(CFU-F)和脾集落形成单位(CFU-S)计数及人源CD45+细胞植入率、归巢示踪等指标。结果采用hUCBDSCs或hBMSCs联合移植造血受抑轻,恢复快,存活率较单移植组(45%)显著提高(P<0.05),分别为75%和70%。尤其以hUCBDSCs共移植组促血小板恢复作用显著加强,并于移植后第21天恢复至照射前水平。hUCBDSCs共移植组在移植后14dCFU-F(70.40±4.34/孔)、移植后21dCFU-F(94.20±8.44/孔)和移植后12dCFU-S计数(34.75±4.50/只)、CD45+细胞植入率(34.19±2.60)%也显著高于其余组(P<0.05)。CM-DiI标记的hUCB-MNCs在体内主要归巢至骨髓和脾脏,并以hUCBDSCs共移植组小鼠骨髓中的含量(85.20±7.89)最多(P<0.05)。结论hUCBDSCs联合hUCB-MNCs共移植能加速小鼠造血重建,提高植入率。
Objective To study the effect of co-transplantation of human umbilical cord blood-derived stromal cells （hUCBDSC） and human umbilical cord blood-mononuclear cells （hUCB-MNC） on hematopoietic reconstruction and engraftment in BALB/c mice with radiation damage.Methods One day after irradiation with 60Coγ at 8.5 Gy,the animals were randomly divided into 4 groups,which were infused with saline,hUCB-MNC（1×107 per mouse）,hUCB-MNC（1×107 per mouse）and hUCBDSC（1×106 per mouse）,and hUCB-MNC （1×107 per mouse）and hBMSC （1×106 per mouse）respectively,through their tail vein.Survival rate,peripheral hemogram,marrow pathological change,CFU-F,CFU-S,engraftment rate,and homing tracing in each group were detected,respectively.Results The survival rate of mice was 75% and 70%,respectively,after co-transplantation of hUCB-MNC and hUCBDSC or hBMSC（P0.05）,which was significantly higher than the only hUCB-MNCs group.The number of platelets was increased in the hUCBDSC plus hUCB-MNC group and became normal 21 d after operation.CFU-F14（70.40±4.34 /well）,CFU-F21（94.20±8.44 /well）,CFU-S12（34.75±4.50 per mouse） and the engraftment rate （34.19±2.60）% were significantly higher in the hUCBDSC co-transplantation group than in other groups （P0.05）.hUCB-MNC labeled by CM-DiI were mainly distributed in bone marrow and spleen,especially in hUCBDSC plus hUCB-MNC group（85.20±7.89）（P0.05）.Conclusion Co-transplantation of hUCBDSC and hUCB-MNCs can significantly promote hematopoietic reconstruction and elevate the engraftment rate.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第9期895-899,共5页
Journal of Third Military Medical University
基金
国家自然科学基金(30971109)
重庆市自然科学基金(CSTC2009BA5011)
重庆市医学重点学科建设基金(2006C028)
第三军医大学新桥医院"1520人才培养工程"基金~~
关键词
人脐血
基质细胞
造血重建
植入
BALB/c小鼠
human umbilical cord
stromal cells
hematopoietic reconstruction
engraftment
BALB/c mice
