摘要
背景:降低不锈钢微螺钉种植体的脱落率,开发新型的抗菌材料,可以从根本上预防种植体周围炎的发生。目的:通过体外细胞培养法评价新型抗菌不锈钢种植体的细胞毒性。方法:将待检试件(抗菌不锈钢、医用不锈钢、医用纯钛)制备成15mm×10mm×3mm的长方体,表面逐级抛光后,无水乙醇脱脂、超声清洗并行高温高压消毒。将各试件以3cm2/mL的比例浸泡于DMEM高糖培养基中置于37℃温箱内96h,微孔滤膜过滤除菌。实验设0.64%的苯酚培养液作为阳性对照组及空白DMEM培养基阴性对照组。倒置相差显微镜观察MG63细胞在各试件浸提液中的生长情况,通过MTT实验得出细胞在培养24,48,72,96,120h后的吸光度值,并计算其相对增殖率评价抗菌不锈钢的细胞毒性。结果与结论:①培养24h,阳性对照组细胞形态异常呈空泡状、其余各组细胞贴壁生长良好。②培养48h后,随着作用时间的延长,阳性对照组细胞表现出明显的中毒形态,其余各组细胞生长旺盛,医用不锈钢及抗菌不锈钢组开始出现少量细胞核固缩。③3组试件吸光度值由高到低依次为医用纯钛、抗菌不锈钢、医用不锈钢,但3者之间差异无显著性意义。④在观察期医用纯钛组、医用不锈钢组、抗菌不锈钢组细胞毒性等级均为0级。结果表明抗菌不锈钢毒性等级与医用不锈钢和医用纯钛相同,符合口腔正畸材料的临床应用要求。
BACKGROUND：Prevention of implant from inflammation was an effective method to reduce expulsion rate of stainless steel micro-screw implant,and develop new type of antibiotic material.OBJECTIVE：To evaluate the cytotoxicity of a new type of antibiotic stainless steels.METHODS：Metal test samples（antibiotic stainless steel,medical stainless steel,and medial pure titanium）were made into rectangular solids with length of 15mm×10mm×3mm.Samples were cleaned with high temperature and high pressure.Alloy leaching liquor was prepared with DMEM culture media according to the ratio between surface area and volume of culture solution（3 cm2/mL）.The leaching liquor was maintained in incubator at 37℃ for 96 hours,and then degerming was performed using microporous membrane.6.4% phenol was added,which was considered as the positive control group,and DMEM culture media was considered as the blank control group.Growth of MG63 cells was observed under inverted phase contrast microscope;absorbance of cells cultured for 24,48,72,96,and 120 hours was detected using MTT test;cytotoxicity of antibiotic stainless steels was evaluated.RESULTS AND CONCLUSION：①At 24 hours after culture,cells in the positive control group was abnormal;while,cells in other groups were well adherent-grew.②After 48 hours of culture,with the culture time increased,cytotoxicity was detected out in the positive control group;cells in other groups and blank control groups were normal and grew well.A few of cells in stainless steels group showed karyopyknosis.③The absorbance was the highest of medical pure titanium,and then of antibiotic stainless steel and of medical stainless steel,while there was no significant difference between the three materials.④The level of cytotoxicity was grade 0.The results suggested that the antibiotic stainless steel which had the same cytotoxicity grade as medical stainless steel and pure titanium was in line with the requirement of its clinical application.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第16期2916-2920,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
