摘要
为探讨出一种能更清楚有效地观察植物木质部结构的细胞学切片方法,以甘蓝型油菜N529及拟南芥野生型col-4为材料,利用快速冷冻切片法、石蜡切片法及徒手切片法制备植物茎组织切片。切片经组织化学染色:1%间苯三酚和25%盐酸处理,并通过Nikon倒置显微镜观察和比较3种不同切片的植物细胞形态结构。结果表明快速冷冻切片法处理的植物细胞结构保存较完好,木质素染色清晰,且操作方法简便、快速、有效。
In order to explore a kind of plant sectioning method which could observe the structure of plant xylem more clearly and effectively,the Brassica napus N529 and Arabidopsis thaliana col-4 were taken as material,and tissue biopsies of plant stem were prepared by rapid cryo-sectioning method,paraffin section method and free-hand section method.Then,these sections were staining by 1% phloroglucinol and 25% hydrochloric acid.The plant cell morphology in these sections were observed and compared through Nikon inverted microscope.The results show that the rapid cryo-sectioning method could relatively preserve the plant cell structure more integrity,the lignin staining clear,and the operation is simple,fast and effective.
出处
《湖南农业科学》
2010年第4期20-22,共3页
Hunan Agricultural Sciences
基金
国家863计划项目(20070349669)
国家自然科学基金(30800080)
关键词
冷冻切片
石蜡切片:徒手切片
cryo-sectioning
paraffin section
free-hand section
