摘要
目的:探讨以jetPEI-RGD为转染载体的125I-(αV)ASODN投递在体外对HepG2细胞侵袭力的影响。方法:125I标记整合素αV亚基的ASODN,以聚乙烯亚胺衍生物jetPEI-RGD为载体,通过受体介导的转染方式进入HepG2细胞,利用Boyden小室侵袭模型评估复合物对HepG2细胞侵袭力的影响,并计算实验组和各对照组的抑制率。结果:jetPEI-RGD/125I-(αV)ASODN组、jetPEI-RGD/(αV)ASODN组、jetPEI-RGD组、125I-(αV)ASODN组、(αV)ASODN组和Na125I组的抑制率分别为(52.60±4.11)%、(39.73±3.40)%、(30.05±5.19)%、(14.14±2.94)%、(12.79±2.68)%和(0.91±2.91)%,相对于其他各实验对照组,jetPEI-RGD/125I-(αV)ASODN组减低了HepG2细胞的侵袭能力(P<0.01)。结论:以jetPEI-RGD为载体投递125I-(αv)ASODN能有效抑制HepG2细胞的侵袭力。
Objective To evaluate the effects of ^125I-（αv） antisense oligodeoxynucleotides （ASODN） delivered by jetPEI-RGD on the invasion ability of human hepatic carcinoma cell line （Hep G2）cuhured in vitro. Methods αv ASODN labeled with ^125I was transfected into HepG2 cells with polyethyleneimine derivatives jetPEI- RGD in a receptor mediated manner. Cell invasion was evaluated by using a Boyden chamber assay, and the inhibition rates of different treatments were calculated. Results The inhibition rates of jetPEI-RGD/^125I-（αv） ASODN、jetPEI-RGD/（αv）ASODN,jetPEI-RGD、^125I-（αv）ASODN, （αv）ASODN and Na^125I on the cell invasion of Hep G2 cells were （52.60 ± 4.11 ）%、 （39.73 ± 3.40）%, （30.05 ±5.19）%, （14.14 ±2.94）%, （12.79 ±2.68）% and （0.91 ± 2.91 ）%,respectively. In contrast to the other experimental groups and control group, jetPEI-RGD/ ^125I-（αv） ASODN treatment significantly reduced the invasive capacity of Hep G2 cells （P 〈 0.01 ）. Conclusion ^125I-（αv） ASODN delivered by jetPEI-RGD effectively reduced the invasion ability of Hep G2 cells.
出处
《实用医学杂志》
CAS
北大核心
2010年第9期1496-1499,共4页
The Journal of Practical Medicine
