摘要
目的:建立C57BL/6小鼠1型糖尿病心肌病模型,并评价该疾病模型的可行性。方法:70只小鼠随机分为模型组(n=43)和对照组(n=27)。模型组小鼠按50mg/kg体重连续5d腹腔注射链脲佐菌素(STZ)溶液,对照组小鼠注射相应体积的柠檬酸缓冲液。每周观察小鼠食量、饮水量、体重、精神活动等基本状况。每2周剪鼠尾取血,检测血糖,评价建模情况。实验第13周处死小鼠,取心脏石蜡包埋,行HE染色、天狼猩红染色显示病理改变,Image-pro plus 6.0分析软件计算胶原面积占总面积比值。结果:模型组小鼠饮水量、食量分别自第2、3周开始高于对照组小鼠(均P<0.01);注射STZ后第2周体重逐渐减轻,低于对照组小鼠(P<0.01)。空腹血糖在建模第1周开始,模型组明显高于对照组(P<0.001)。HE染色及天狼猩红染色显示模型组小鼠心肌纤维排列紊乱,分布不均,肌细胞间质明显增加。结论:本实验成功建立了C57BL/6小鼠1型糖尿病心肌病模型,该模型可行性强,稳定性好。
Objective To establish a murine model of type 1 diabetic cardiomyopathy in C57BL/6 mice, then evaluate the feasibility of this model. Methods 70 C57BL/6 mice were randomized to receive either intraperitoneal injection of streptozotocin （STZ） solution for 5 days （model group,n = 43） or the same volume of citric acid buffer （control group,n = 27）. The intake of food and water, body weight, and mental activities were observed weekly. Blood suger was detected every 2 weeks to assess the establised model. The pathological changes in the mouse heart were determined using HE staining and Sirius red staining on weeks 13. The ratio of collagen area to total area was calculated by Image-Pro Plus 6.0. Results Food intake started to be greater on week 2 and water intake to be greater on week 3 in the model group than in the control group （P 〈 0.01 for both comparisons） ; body weight was lower in the model group than in the control group 2 weeks after injection of STZ （P 〈 0.01 ）. Fasting blood glucose was significantly higher in the model group than in the control group on week 1 （P 〈 0.001 ）. HE staining and Sirius red staining showed that myocardial fibers were disarranged and distributed unevenly; interstitial substances increased significantly. Conclusions A murine model of type 1 diabetic cardiomyopathy is successfully established in C57BL/6 mice. This model is highly feasible and has good stability.
出处
《实用医学杂志》
CAS
北大核心
2010年第9期1518-1520,共3页
The Journal of Practical Medicine
关键词
糖尿病
1型
心肌疾病
链脲佐菌素
Diabetes mellitus, type 1
Cardiomyopathies
Streptozotocin
