摘要
目的:利用Fourier变换红外光谱(FT-IR)法结合化学计量学方法对黄芪药材进行产地鉴别。方法:提出一种溶剂选择的评价方法,对5个不同产地86个黄芪药材样本采用单溶剂进行提取,然后将提取物以KBr涂膜进行红外光谱扫描测定。采用偏最小乘判别法(DPLS)对黄芪药材进行产地鉴别,并用变量投影重要性指标(PLS-VIP)对鉴别有意义的波数进行选择。结果:单溶剂提取法中丁酮提取物具有较好的光谱学特征,黄芪药材产地鉴别的正确率为100%,选择的波数包含了对鉴别有价值的信息。结论:红外光谱法结合化学计量学方法能够用于黄芪药材的产地鉴别。
OBJECTIVE：To identify the habitat of Astragalus membranaceus by Fourier transform infrared spectroscopy and chemometrics methods.METHODS：An evaluation method for selecting solvent applied to extract 86 samples from 5 different habitats as a pretreatment method was proposed.IR spectra were used to detect samples coated with KBr.Partial least squares （PLS） were used to identify the habitats of A.membranaceus.Significant IR spectral wave numbers were selected by PLS-VIP score.RESULTS：In single-solvent extraction method butanone extract had IR spectra characteristics.The incidence of successful identification was 100% and selected IR spectral wave were valuable for identification.CONCLUSION：FT-IR spectroscopy combined with chemometrics method can be used to identify the habitats of A.membranaceus.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第19期1772-1774,共3页
China Pharmacy
基金
山东省自然科学基金项目(Q2006C05)
关键词
红外光谱法
化学计量学
黄芪
溶剂提取
产地鉴别
Infrared spectroscopy
Chemometrics method
Astragalus membranaceus
Solvent extraction
Habitat identification
