摘要
目的通过对外周血单核细胞（ＰＢＭＣ）中ＨＣＶＲＮＡ正负链的检测，来探讨其与丙型肝炎慢性化及干扰素治疗的关系．方法慢性丙型肝炎患者４０例，其中干扰素治疗者１０例，分离血清及ＰＢＭＣ．异硫氰酸胍－酚－氯仿抽提法提取ＨＣＶＲＮＡ，应用逆转录－巢式ＰＣＲ技术检测ＨＣＶＲＮＡ正负链．结果血清正链ＨＣＶＲＮＡ阳性率为６７５％，但负链均为阴性，ＰＢＭＣ中正链ＨＣＶＲＮＡ阳性率为５７５％，负链的阳性率为３５０％．其中３例患者血清中正链ＨＣＶＲＮＡ为阴性，而ＰＢＭＣ中为阳性．１０例干扰素治疗者在治疗结束时血清正链ＨＣＶＲＮＡ６０％转阴，ＰＢＭＣ中负链ＨＣＶＲＮＡ８０％转阴，而正链仅３７５％转阴．结论ＨＣＶ能在ＰＢＭＣ中存在和复制，这可能是导致丙型肝炎易发生慢性化的原因之一．
AIM To study the relation between chronic hepatitis C and interferon therapy with the positive and negative strands of HCV RNA in peripheral blood mononuclear cells(PBMC). METHODS The sera and PBMC of forty patients with chronic hepatitis C were separated. HCV RNA was extracted by acid guanidinium thiocyanate phenol chloroform. HCV RNA was detected with reverse transcription and nested polymerase chain reaction. RESULTS Positive strands of HCV RNA were detected in 67 5 percent of sera and all negative strands in sera were negative. Positive rates of positive and negative strands of HCV RNA in PBMC were 57 5 percent and 35.0 percent. In three patients, the positive strands of HCV RNA were not detected in sera, but were detected in PBMC. When the IFN therapy was finished, 60 percent positive strands in sera became negative, while 37 5 percent positive strands and 80 percent negative strands in PBMC became negative. CONCLUSION HCV can infect PBMC and replicate in these cells, which might be one of the causes of chronic hepatitis C. The detection of positive and negative strands of HCVRNA in PBMC is very important in evaluating the curative effect of IFN therapy and prognosis of the patients.
出处
《世界华人消化杂志》
CAS
1999年第3期220-221,共2页
World Chinese Journal of Digestology
