摘要
采用基因重组的方法构建了含有猪传染性胃肠炎病毒S基因B和C抗原位点片段的巴斯德毕赤酵母Pichiapastoris分泌型表达载体pPIC9K-ts,经线性化后采用电穿孔法将其导入毕赤酵母GS115中,大量筛选后获得高效表达外源蛋白的毕赤酵母工程菌株GS115/pPIC9K-ts。表达蛋白经Dot-ELISA检测具有良好的抗原性。本研究为TGE的血清学检测方法的建立提供了必要的物质基础。
A DNA fragment coding for TGEV spike gene B and C antigen sites was cloned into P. pastoris expression vector pPIC9K for extraeellular expression in P. pastoris by gene recombination. The resulted recombinant plasmid pPIC9-ts was then linearized and transformed into P. pastoris GS115 by electroporation. The ts gene was integrated into the genome of P. pastoris through homologous recombination and an expression strain, named GS115/pPIC9K-ts, was obtained. Dot-ELISA showed that the recombinant protein possessed favourable antigenicity. This research provided essential material foundation for establishment of TGE serology diagnostic method.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第5期154-157,共4页
Biotechnology Bulletin
基金
黑龙江省"九五"攻关课题(G00B03021)
