摘要
以超声萃取-酸水解-超声萃取进行预处理,结合RP-HPLC测定黄姜中薯蓣皂甙元含量。色谱条件为:采用AglilengtHC-C18色谱柱,流动相为甲醇∶水=95∶5(V∶V),流速为1.0mL/min,UV检测波长为203nm。结果表明,在40—1000μg/mL范围内,薯蓣皂甙元峰面积与其质量浓度具有良好的线性关系(r=0.9996),样品平均加标回收率为99.8%,RSD为1.1%。与直接酸水解-萃取预处理方式进行对比,本方法测得薯蓣皂甙元含量提高了32%。采用本方法对黄姜芽,根茎,表皮及不定根中的薯蓣皂甙元含量进行测定:根茎1.69%,芽0.19%,表皮0.15%,不定根部分含量为0.36%。
An improved pretreatment（ultrasonic extraction-acid hydrolysis-ultrasonic extraction） coupled with RP-HPLC was promoted for determination of diosgenin in Dioscorea zingiberensis.Chromatography conditions were as follows：Aglilengt HC-C18（4.6mm×250mm,5μm）column was used with temperature of 35℃;the mobile phase consisted CH3OH-H2O（95：5,V：V）;diosgein was determined at 203nm,at flow rate of 1.0mL/min.The results indicated that the peak area of diosgenin was linearly correlated to the diosgenin concentration within the range of 40—1000μg/mL.The calibration curve showed good linear regression （r=0.9996）,and the average recovery was 99.8%,the RSD was 1.1%.The content of diosgenin was increased 32% with this pretreatment compared with direct acid hydrolysis-extraction.The described method was applied for determination of diosgenin in the rhizome,rootage,peel and sprout of Dioscorea zingiberensis C.H.Wright.As a result,the content of diosgenin was 1.69% in rhizome,0.15% in peel,0.19% in sprout and 0.36% of rootage.
出处
《光谱实验室》
CAS
CSCD
北大核心
2010年第3期961-965,共5页
Chinese Journal of Spectroscopy Laboratory
基金
国家科技支撑计划"丹江口水源区黄姜加工新工艺关键技术研究"(2006BAB04A14)
湖北省科技厅重大科技攻关项目"年产300吨黄姜皂素循环经济生产系统及工程示范"(2006AA305A05)
