摘要
目的:对藿香正气水样品前处理的中国药典方法进行改进,建立采用SPE-HPLC法测定藿香正气水中厚朴酚与和厚朴酚含量的分析方法。方法:采用自制硅胶SPE小柱提取纯化样品。采用C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水-乙腈-冰醋酸(70:25:5:0.25)为流动相,流速1.0mL.min-1,检测波长294nm,柱温为室温。结果:SPE-HPLC法测得厚朴酚浓度在4.0～80μg.mL-1(r=0.9998),和厚朴酚浓度在2.0～40μg.mL-1(r=0.9998)范围内与其峰面积线性关系良好;厚朴酚的平均回收率(n=5)为99.9%(RSD=0.7%),和厚朴酚的平均回收率(n=5)为99.5%(RSD=0.5%)。结论:该方法环保、简便、准确,重现性好,能有效地控制藿香正气水的质量。
Objective：Based on the improvement of the sample pretreatment method in the criterion,an SPE-HPLC method for determining the contents of magnolol and honokiol in Huoxiang Zhengqi solution was established.Methods：Samples were purified by silica gel SPE column.The HPLC column used in the experiment was C18（250 mm×4.6 mm,5 μm）,and the mobile phase was methanol-water-acetonitrile-glacial acetic acid（70：25：5：0.25）at the flow rate of 1.0 mL·min-1;The detection wavelength was 294 nm,and the experiment was accomplished at room temperature.Results：The contents of magnolol and honokiol determined by SPE-HPLC method had good linear relationship with the peak areas when their concentrations at 4.0-80.0 μg·mL-1（r=0.9998）,2.0-40.0 μg·mL-1（r=0.9998）,respectively;The average recovery（n=5）of magnolol was 99.9%（RSD=0.7%）,and that of honokiol was 99.5%（RSD=0.5%）.Conclusion：The method is environmental protection,simple,accurate,with good reproducibility,and it can be used for the quality control of Huoxiang Zhengqi solution.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第5期827-830,共4页
Chinese Journal of Pharmaceutical Analysis
基金
河北大学自然科学基金资助项目(2007-111)
