摘要
以7、8、9世代京海黄鸡母鸡为试验材料,采用PCR-SSCP技术对IGF-Ⅰ基因外显子3多态位点进行研究,并计算基因型、基因频率、卡方值和部分遗传多态性指标。结果表明,A等位基因随着世代数的增加不断减少;3个世代之间基因型分布达到显著(P<0.05)或极显著(P<0.01)差异;遗传杂合度随世代数的增加而升高;该位点3个世代均为中度多态。初步推断选留的确对IGF-Ⅰ基因31位点造成了影响,该位点可应用于育种实践中。
Genetic diversity of exon 3 in insulin-like growth factor-Ⅰ（IGF-Ⅰ） gene was studied using PCR-SSCP technique in seven,eight and nine generations of Jinghai yellow chicken population in the present study,and calculated the genotype,gene frequency,chi-square value and some indicators of genetic polymorphism. The results showed that alleles increased with decreasing the number of generations; genotype distribution between the three generations achieved a significant（P〈0.05） or very significant（P〈0.01） difference; heterozygosis increased with the number of generations increased; the locus polymorphism of three generations were moderate. Indeed,the initial inference elected to stay on the IGF-Ⅰ gene had an impact on 31 points,the loci could be applied in breeding practice.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第5期126-129,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家肉鸡产业技术体系资助(nycytx-42-G1-05)
