摘要
ABF/AREB/ABI5/DPBF类转录因子属于碱性亮氨酸类蛋白,在依赖ABA的逆境信号传导途径中起重要的作用。本研究结合该类转录因子的结构特点,利用3′RACE技术和优化的TAIL-PCR技术成功获得棉花中三个该家族成员编码基因。进一步验证结果表明两步PCR成功获得基因完整的ORF、3′UTR以及部分5′UTR序列。本研究所获基因为棉花抗逆基因工程提供了候选基因源,优化后的TAIL-PCR技术为克隆棉花中目的基因提供了一种简便高效的方法。
ABF/AREB/ABI5/DPBF transcription factors belong to basic leucine zipper protein,this kind of transcription factors plays an important role in the signal transduction pathways of ABA-dependent abiotic stress.In this study,taking structure char-acteristics of ABF/AREB/ABI5/DPBF as the research object,we cloned three bZIP encoding genes by 3′ RACE successfully from cotton and also optimized the TAIL-PCR conditions.The further experiment results showed that we had got the integrated sequences of the ORF,3′ UTR,as well as part of 5′ UTR sequence.The genes we cloned will provide candidate genes for resis-tance genetic engineering of cotton.Furthermore,the optimized methodology of TAIL-PCR will provide an easy and efficient technology for cloning of target genes.
出处
《棉花学报》
CSCD
北大核心
2010年第3期195-201,共7页
Cotton Science
基金
农业部国家转基因重大专项:"转基因抗旱耐盐碱棉花新品种培育"
2008ZX005-004
