摘要
检测了编号为M1、M2、M7、M8、10#羊肚菌菌丝体在液体培养基中生长时胞外酶的活性及培养液中糖与蛋白质的含量，同时观察了菌丝体生长情况。结果表明：菌丝体生长情况各不相同，10#在菌丝片的产生及菌丝成团最早，依次为10#〉M8〉M7=M2〉M1；与碳水化合物降解相关的酶（纤维素酶、淀粉酶）活性变化趋势基本相同，在发酵的第2～3天出现第1个峰值，并于第6天出现第2个峰值，且第1个峰值大于第2个峰值；在整个培养期内漆酶、愈创木酚酶、蛋白酶均有活性，产酶高峰也不尽相同；蛋白质、糖含量随时间变化且与酶活力相关。
Activities variations of five extracellular enzymes, protein content, and sugar content from Morchella esculenta by liquid culture were studied. And the relation ship between mycelium growth of Morckella esculentaand activities of the enzymes was investigated. The results showed that mycelium growth of different, 10 hyphae into the first group followed by10#〉M8〉M7 = M2〉M1 ;The changing trends of activities of the enzymes degrading carbohydrate were similar basically. Activities of all the enzymes showed two peak values during the whole fermentation, and the first peak valueis higher than the second one. During the whole culture the three enzymes had activities. And their production peaks werealso different. Protein and sugar content change over time and with the enzyme activity-related.
出处
《北方园艺》
CAS
北大核心
2010年第10期210-213,共4页
Northern Horticulture
基金
延安大学专项科研基金资助项目(YDK2008-17)
关键词
羊肚菌
菌丝体
液体培养
胞外酶
Mochella
mycelium
liquid culture
exocellular enzyme
