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辣椒素对宫颈癌Hela细胞增殖影响的研究 被引量:9

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摘要 目的:探讨TRPV1通道在宫颈癌Hela细胞的表达以及辣椒素对Hela增殖的影响。方法:RT-PCR和Western blot检测TRPV1通道在Hela细胞的表达。MTT法和流式细胞术检测辣椒素对Hela细胞增殖,以及细胞周期和凋亡的影响。通过荷瘤鼠动物模型探讨辣椒素对体内宫颈癌生长的影响。结果:辣椒素能抑制Hela细胞的增殖,其作用随辣椒素浓度增加而增强,其机制是通过激活TRPV1通道引起细胞内Ca2+浓度升高,诱导细胞死亡;体内试验表明辣椒素能显著抑制肿瘤生长[(575.02±41.83)mm3vs(659.80±59.42)mm3,P<0.01]。结论:Hela细胞表达有功能的TRPV1通道;辣椒素能通过激活TRPV1通道而诱导细胞死亡和抑制体内宫颈癌瘤体生长,具有潜在的治疗价值。
出处 《广西医科大学学报》 CAS 2010年第2期213-215,共3页 Journal of Guangxi Medical University
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