摘要
目的了解姜黄素和TSA对胃癌c-myc基因表达影响,探讨组蛋白乙酰化/去乙酰化的动态平衡在胃癌基因表达和调控中的意义。方法进行胃癌细胞株SGC-7901和MGC803培养,然后加入不同浓度的组蛋白乙酰化酶抑制剂姜黄素和组蛋白去乙酰化酶抑制剂TSA分别进行干预,RT-PCR检测c-myc的mRNA的表达。结果通过RT-PCR的检测我们发现随着姜黄素的浓度增加及TSA浓度的减小c-myc的表达受到抑制(P<0.01)。结论姜黄素与TSA对c-myc表达影响作用是相反的。
Objective To understand the effect of curcumin and TSA on c - myc gene expression in human gastric cancer and to explore the dynamic balance of the histone acetylation / deacetylation in gene expression in gastric cancer and the significance of the regula- tion. Methods Gastric cancer cell line SGC -7901 and MGCS03 were cultured, and then different concentrations of histone acetylase inhibitors curcumin and histone deacetylase inhibitor TSA were added, respectively, mRNA of e -myc expression wasdeteeted by RT - PCR. Results With increasing of the concentration of curcumin and redcuing of the TSA concentrations c - myc expression was inhibited （P 〈 0.01 ）. Conclusion The effect of curcumin and TSA on c - Myc expression is contrary.
出处
《医学研究杂志》
2010年第5期82-83,共2页
Journal of Medical Research
