摘要
目的 探讨儿童氟斑牙发生与雌激素受体基因α（ERα）Xba I多态性的关系.方法 于2006年选取河南省开封县仇楼乡、通许县孙营乡作为调查点,每个点选饮水型地方性氟中毒病区（高氟区）、对照区各1个,对调查区的8～12岁儿童进行氟斑牙检查,氟斑牙诊断采用Dean法,并依据高氟区儿童氟斑牙患病情况分为氟斑牙组、病区对照组;对照区儿童为非病区对照组.利用聚合酶链反应-限制性片段长度多态性（PCR-FRLP）方法检测儿童ERα Xba I基因型,氟离子选择电极法测定尿氟,计算尿氟超标率.结果 在高氟区儿童氟斑牙检出率为51.7%（74/143）,氟斑牙指数为1.310;在对照区共调查94人,未检出氟斑牙患者,氟斑牙指数为0.021;高氟区儿童尿氟超标率[84.6%（121/143）]明显高于非病区对照组[9.6%（9/94）;χ^2125.95,P〈0.01].ERα Xba I基因型XX、Xx、xx分布频率,氟斑牙组分别为6.8%（5/74）、36.5%（27/74）、56.8%（42/74）,病区对照组分别为15.9%（11/69）、37.7%（26/69）、46.4%（32/69）,非病区对照组分别为14.9%（14/94）、43.6%（41/94）、41.5%（39/94）,ERα Xba I基因型在3组儿童中分布组间比较差异无统计学意义（χ^23.450,P〉0.05）.尿氟超标儿童ERα Xba I等位基因X、x频率,氟斑牙组儿童分别为22.7%（30/132）、77.3%（102/132）,病区对照组儿童分别为35.5%（39/110）、64.5%（71/110）,二者比较差异有统计学意义（χ^2=4.768,P〈0.05;OR=0.535.95%CI：0.305～0.941）.结论 在尿氟超标的儿童中,携带X等位基因可能降低氟斑牙发生的危险性.
Objectives To explore the relationship between polymorphism in estrogen receptor alpha （ERα）gene Xba I and child dental fluorosis.Methods Qiulou township of Kaifeng and Sunying township of Tongxu counties of Henan province were chosen as the investigation spots in 2006.An area of water drinking endemic fluorosis（high fluoride area）and a non-endemic area（control area）were chosen in every spot,where dental fluorosis of children aged 8 to 12 years old were examined and diagnosed by using the Dean method.The children in the high fluoride areas were divided into dental fluorosis group and control group of the endemic areas according to dental fluorosis status,and the children in the control areas as control gruop of non-endemic areas.The Xba I polymorphism in the ERα gene was genotyped using the PCR-RFLP procedure.The fluoride levels in the urine samples from the three groups were detected by fluoride ion selective electrode and over standard rate of the urine was counted.Results The prevalence rate of dental fluorosis in high fluoride areas was 51.7%（74/143）and the community fluorosis index was 1.310.No dental fluorosis case was checked out in the control and the community fluorosis index was 0.021.The over standard rate of urine fluoride in dental fluorosis group[84.6%（121/143）]was significantly higher than that of control in non-endemic area[9.6%（9/94）;χ^2125.95,P〈0.01].The frequency distribution of ERα Xba I genotype was XX 6.8%（5/74）,xx 36.5%（27/74）,xx 56.8%（42/74）in dental fluorosis group;XX 15.9%（11/69）,Xx 37.7%（26/69）,xx 46.4%（32/69）in the eontrol of the endemic areas;XX 14.9%（14/94）,Xx 43.6%（41/94）,xx 41.5%（39/94）in children from the control in non-endemic area,respectively.No significant difference was found among the three groups（χ^2 3.450, P 〉 0.05）. Allele frequency of ERα Xba I genotypes was X 22.7%（30/132）, x 77.3%（102/132） in dental fluorosis group and X 35.5%（39/110）,x 64.5% （71/110） in the control in endemic area when urine fluorosis of children was exceeding standard and significant difference was found in this two groups（χ^2= 4.768, P 〈 0.05; OR = 0.535,95% CI：0.305 - 0.941）. Conclusion Children who carried X allele frequency of ERα Xba I genotypes have a lower risk of dental fluorosis when children with high-loaded fluoride status.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2010年第3期278-281,共4页
Chinese Jouranl of Endemiology
基金
河南省教育厅自然科学基金（2009A330005）
郑州市国际合作项目（083SGHH33292-2）
关键词
雌激素受体Α
多态性
基因
氟中毒
牙
Estrogen receptor alpha
Polymorphism, genetic
Fluorosis, dental
