摘要
目的探讨脉络宁注射液对大鼠脑缺血-再灌注损伤的治疗作用以及可能的机制。方法将54只SD大鼠按随机数字表法分为假手术组、缺血-再灌注组(IR组)及治疗组,每组各9只。造模后6 h进行神经功能缺损评分。治疗组分别在不同时间点(6、24、48 h和7 d)经尾静脉注射脉络宁注射液(40 g·kg^(-1) ·d^(-1)),连用7 d。注射体积为1.5 ml。假手术组及IR组在相应时间点给予等体积的等渗盐水。疗程结束后(第14天)进行神经功能缺损评分、取材。电镜观测海马CA1区细胞形态,免疫组化检测血管内皮生长因子阳性细胞。结果①第14天疗程结束后,治疗组6、24、48 h和7 d亚组大鼠神经功能缺损评分分别为3.2±0.8、3.3±0.5、3.5±0.8、4.5±0.8,均低于IR组评分5.5±1.1;而治疗组48 h及48 h以内给药亚组大鼠的神经功能缺损评分低于7 d亚组。差异均有统计学意义(P<0.05)。②治疗组(6、24、48 h和7d亚组)大鼠海马超微结构较IR组明显改善,其中6 h亚组恢复最好。③治疗组(6、24、48 h和7 d亚组)大鼠脑组织血管内皮生长因子(VEGF)阳性细胞率分别为(19.4±1.3)%、(17.6±1.3)%、(17.2±1.5)%和(12.6±1.6)%,均高于IR组的(6.1±1.3)%,差异有统计学意义(P<0.01)。尤以6 h亚组VEGF的表达水平最高,为(19.4±1.3)%。结论脉络宁注射液对大鼠脑缺血-再灌注损伤早期具有较好的治疗作用,48 h以内给药效果更好,可能通过增加血管内皮生长因子的表达而发挥作用。
Objective To investigate the therapeutic effect of mailuoning injection on cerebral ische- mia-reperfusion injtiry in rats and its possible mechanisms. Methods Fifty-four SD rats were randomly divided into sham operation group, ischemia-reperfusion （IR） group and treatment group （n = 9 in each group）. Functional score was performed at 6 hours after modeling. Mailuoning 1.5 mL （40 g · kg-1 · d-1） was injected at different time points （6, 24, 48 h, and 7 d） respectively via tail veins for 7 days in the treatment group. The equal volume of isotonic saline was given at the corresponding time points in the sham operation and IR groups. The rats were scored and sampled after the end of treatment （ at days 14）. The cell morphology in the hippocampal CA1 region was observed with electron microscopy, and immunohis- tochemistry was used to detect vascular endothelial growth factor （VEGF） positive ceils. Results （1）The neurological scores in the 6, 24, 48 h, and 7 d groups were 3.2 ± 0. 8, 3.3 ± 0. 5, 3.5 ± 0. 8 and 4. 5 ± 0. 8, respectively, and they all lower than those in the IR group （5. 5 ± 1.1 ） ; and the neurological scores within 48 h in the treatment group were lower than those in the day 7 group. There were significant differ- ences （ P 〈 0.05 ）. （2）The ultrastructure of rat hippocampus in the treatment group （6, 24, 48 h, and 7 d groups） was improved more significantly than that in the IR group, and the 6 h group recovered best. （3）The proportions of VEGF-positive cells in rat brain tissue in the treatment group （6, 24, 48 h, and 7 d groups） were 19. g ± 1.3%, 17. 6 ± 1.3%, 17. 2 ±1.5%, and 12. 6 ± 1.6%, respectively, and they were all higher than 6. 1 ± 1.3% in the IR group. There were statistically significant differences （P 〈0. 01 ）, especially the expression level of VEGF in the 6 h group was the highest （ 19.4 ± 1.3% ）. Conclusion Mailuoning injec- tion has better therapeutic effect on early cerebral ischemia-reperfusion injury in rats. The efficacy is better when given within 48 h, and it may play a role by increasing the expression of VEGF.
出处
《中国脑血管病杂志》
CAS
2010年第5期258-262,共5页
Chinese Journal of Cerebrovascular Diseases
关键词
脑缺血
再灌注损伤
血管内皮生长因子类
大鼠
脉络宁注射液
Brain ischemia
Reperfusion injury
Vascular endothelial growth factors
Rats
Mailuoning injection
