摘要
目的:建立尿中三氯乙酸的捕集阱顶空-气相色谱测定方法。方法:尿中的三氯乙酸加热脱羧生成三氯甲烷,经顶空提取并被捕集阱吸附,解析后进气相色谱分离,电子捕获检测器检测。结果:三氯乙酸在1μg/L～100μg/L浓度范围内线性良好(r>0.999),最低检出浓度为1×10-3μg/L,标准溶液测定的相对标准偏差(relative standard deviation,RSD)为0.64%～2.30%,加标回收率为86.5%～108.4%。结论:本法简单灵敏,为测定尿中三氯乙酸的人群正常值提供了技术支持。
Objective：To determine trichloroacetic acid in urine,a method of headspace gas-chromatography with adsorption trap was established.Methods：After the trichloroacetic acid in urine decarboxylated and formed into chloroform which was collected by headspace with adsorption trap,the chloroform was determined by capillary gas chromatography with ECD.Results：There was a good linearity when the concentration of trichloroacetic acid was 1 μg/L～100 μg/L（r0.9990）.The limit of detection was 1×10^-3 μg/L（v=10 ml） and the RSD of standard solution 0.64%～2.30%.The proposed method was applied for analysis of urine samples and obtained the satisfactory results and the recovery was 86.5%～108.4%.Conclusion：The method is simple and sensitive.It can provide an important technical support for determination of trichloroacetic acid in urine for normal population.
出处
《中国卫生检验杂志》
CAS
2010年第5期1042-1043,共2页
Chinese Journal of Health Laboratory Technology
基金
国家十一五课题(2006BAI06B02)基金资助
关键词
顶空气相色谱
捕集阱
尿
三氯乙酸
Adsorption trap
Headspace gas chromatography
Urine
Trichloroacetic acid
