摘要
目的:探讨败血症患儿血浆降钙素原(PCT)和白细胞介素-6(IL-6)的变化及其临床意义。方法:应用双抗夹心免疫法(ILMA)测定血浆PCT水平,酶联免疫(ELISA)双抗体夹心法测定血浆IL-6水平。结果:新生儿败血症组血浆PCT和IL-6急性期水平分别为15.6±7.2μg/L和254.9±53.5 ng/L,恢复期二项指标分别为1.1±0.4μg/L和70.3±15.2 ng/L,对照组二项指标分别为0.9±0.3μg/L和65.0±13.7 ng/L,新生儿败血症组PCT和IL-6水平急性期明显高于恢复期,急性期明显高于对照组(P<0.01)。结论:血浆PCT和IL-6可作为新生儿败血症快速有效诊断参考指标和评估病情的检测指标。
Objective：To study the changes and clinical significance of plasma procalcitonin（PCT） and interleukin-6（IL-6） in neonatal sepsis.Methods：Application of double-antibody sandwich immunosorbent assay（ILMA） to test plasma PCT level and enzyme-linked immunosorbent（ELISA） double antibody sandwich method to test plasma IL-6 level.Results：The levels of plasma PCT and IL-6 in septic acute stag group were 15.6±7.2 μg/L and 254.9±53.5 ng/L,their levels in septic restoration stag group were 1.1±0.4 μg/L and 70.3±15.2 ng/L,their levels in control group were 0.9±0.3 μg/L and 65.0±13.7 ng/L.The levels of plasma PCT and IL-6 in septic acute stage group were significantly higher than that in restoration stage,they were significantly higher in acute stage than that in control group（P〈0.01）.Conclusion：Plasma PCT and IL-6 could be used as rapid diagnostic markers for neonatal sepsis.
出处
《中国卫生检验杂志》
CAS
2010年第5期1153-1154,共2页
Chinese Journal of Health Laboratory Technology
