摘要
目的 观察CPB诱发犬急性肺损伤时肺组织转化生长因子β1（TGF-β1）mRNA表达的变化.方法 成年健康杂种犬36只,体重15～16 kg,随机分为2组（n=18）：对照组（C组）和CPB组.CPB组制备CPB诱发急性肺损伤模型,C组除不进行CPB外,其余操作同CPB组.分别于CPB前（T0）、CPB停机后30 min（T1）、60 min（T2）时各处死6只犬,取肺组织,光镜下观察肺组织病理学结果,计算肺通透性指数（PPI）,测定肺组织TGF-β1 mRNA表达和MDA含量.结果 C组肺组织结构清晰,未见炎性细胞浸润,肺泡壁光滑、完整,肺泡腔及间质无出血和渗出;CPB组肺泡腔内可见水肿液和中性粒细胞浸润,并可见大量的红细胞,肺泡隔增宽,局灶性肺不张.与C组比较,CPB组TGF-β1 mRNA表达上调,MDA含量和PPI升高（P〈0.05或0.01）.CPB组TGF-β1 mRNA表达水平与MDA含量和PPI呈正相关（MDA：r=0.867,P〈0.01;PPI：r=0.821,P〈0.01）.结论 犬CPB后肺组织TGF-β1 mRNA 表达上调,可能是CPB诱发急性肺损伤发生发展的重要因素之一.
Objective To observe the changes in the transforming growth factor-beta 1 （TGF-β1 ） mRNA expression in the lung in a dog model of cardiopulmonary bypass（CPB）-induced acute lung injury. Methods Thirty-six healthy adult mongrel dogs of both sexes weighing 15-16 kg were randomly assigned into control group and CPB group （ n = 18 each） . Lung injury was produced by CPB according to the method described by Williams. Six animals were killed at each of the following time points： before CPB （T0 ） and 30 and 60 min after termination of CPB （T1 , T2） in each group. Lung specimens were obtained for microscopic examination and determination of TGF-β1 mRNA expression （by RT-PCR） and MDA content. The lungs were lavaged and the protein concentration in the brancho-alveolar lavage fluid （BALF） was determined and pulmonary permeability index （PPI） was calculated. Results Microscopic examination showed massive inflammatory cell infiltration, alveolar capillary dilatation, congestion, widened alveolar septum, massive RBC in the alveolar space and focal atelectasis in the lung in CPB group. The TGF-β1 mRNA expression and MDA content and PPI were significantly higher in CPB group than in control group. The TGF-β1 mRNA expression and MDA was positively correlated to PPI （MDA： r = 0.867, P 〈 0.01; PPI： r = 0.821, P 〈 0.01） . Conclusion TGF-β1 mRNA expression in the lung is significantly up-regulated after CPB and is an important factor contributing to CPB-induced acute lung injury.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第3期354-357,共4页
Chinese Journal of Anesthesiology
基金
浙江省卫生厅医药卫生科学研究基金（2008B20508）
关键词
转化生长因子Β
心肺转流术
呼吸窘迫综合征
成人
Transforming growth factor beta
Cardiopulmonary bypass
Respiratory distress syndrome,adult
