摘要
从采集的土样中分离筛选11株对冬枣细交链格孢病菌、多隔镰孢霉和美澳核果褐腐串珠霉具有较好抑制作用的拮抗菌。通过平板对峙生长法测抑菌半径。结果表明:其中B26的抑菌效果最显著,对细交链格孢的平均抑菌圈半径为15.8mm,抑制率达78.8%,对冬枣多隔镰孢霉和美澳核果褐腐串珠霉的平均抑菌圈半径分别为14.3mm和12.8mm,抑制率分别达71.3%和63.8%。通过在PDA生长的形态学观察、生理生化实验以及PCR产物测序结果综合分析,初步鉴定B26为枯草芽孢杆菌。
Eleven strains of antagonistic bacteria were isolated from soil samples and exhibited an excellent antagonistic effect on Alternaria alternate,Fusarium decemcellulare Brick and Monilia fructicola.Results showed strain B26 had the strongest inhibition effect on three selected fungal pathogens.The average radius of inhibition zone was 15.8 mm in plate inhibition test and inhibition rate against Alternaria alternate was 78.8%.Similarly,strain B26 also exhibited inhibition effect on Fusarium decemcellulare Brick and Monilia fructicola with average radius of 14.3 mm and 12.8 mm,and inhibition rate of 71.3 % and 63.8%,respectively.Strain B26 was primarily identified as Bacillus subtilis through the characterization of morphological features,cultural characteristic observation PDA,physiological and biochemical measurements and 16S rDNA sequence analysis of PCR products.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第9期150-155,共6页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAD01A16)
山西省青年基金项目(2008021036-1)
山西省留学人员科研项目(2008-119)
太原市科技局项目(2008)
关键词
冬枣
病害
拮抗菌
筛选
鉴定
Jujube fruit
post-harvest diseases
antagonistic bacteria
screening
identification
