摘要
目的:探讨IL-7基因转染人舌鳞癌Tca8113细胞株的免疫调节效应。方法:将已构建的原核/真核表达载体pBK-CMV/IL-7转染到癌细胞株中,采用MTT法检测转染前后肿瘤细胞培养上清对鼠脾细胞增殖的影响;ELISA法检测细胞培养上清转移生长因子β1(TGF-β1)、血管内皮生长因子(VEGF)和IL-10的浓度。将pPK-CMV/IL-7癌细胞株移植于小鼠腹腔,观察肿瘤细胞的成瘤性;并采用MTT法检测其NK杀伤活性。结果:IL-7基因转染癌细胞株的培养上清对鼠脾细胞的增殖抑制作用与对照组比较显著下降(P<0.05),并且IL-7基因转染的肿瘤细胞产生TGF-β1、VEGF和IL-10三种免疫抑制因子明显降低(P<0.05);IL-7基因转染组鼠腹腔瘤结节明显小于对照组(P<0.05);NK的杀伤活性与对照组比较,具统计学差异(P<0.05)。结论:IL-7基因转染人舌鳞癌细胞株具有抑瘤作用,其机理可能是通过降低肿瘤细胞产生免疫抑制因子,进而促进淋巴细胞增殖,提高NK的杀伤活性。
AIM： To investigate the effect of immune regulation of IL-7 gene transfected into human carcinoma line Tca8113. METHODS： pBK-CMV/IL-7 plasmid was transfected into human tongue squamous carcinoma cell lines. The effect of the supernatant of carcinoma culture on the proliferation of lymphocytes was measured using MTT method. The concentrations of TGF-β1, VEGF and IL-10 were determined by ELISA. Human carcinoma cells lines with pPK-CMV/IL-7 were injected into peritoneal of mice, and the tumor formed in peritoneal was detected. The killing activity of NK was analyzed by MTT. RESULTS： Compared with control group, the supernatant from carcinoma cells culture could significantly suppress the proliferation of lymphocytes （P〈0.05）. The supernatant levels of TGF-β1, VEGF and IL-10 were obviously decreased. The tumor volume in experimental group were fewer then those in control group（P〈0.05）. The killing activity of NK was significantly increased （P 〈0.05）. CONCLUSION： IL-7 gene transfection into human carcinoma line Tea8113 could inhibit tumor growth by reducing secretion of immunosuppressive factors, enhancing lymphocyte proliferation and increasing the killing activity of NK.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2010年第3期247-250,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
中国-意大利科技合作项目(200625)
关键词
IL-7
免疫调节
口腔癌细胞株
IL-7
Immune regulation
Human tongue squamous carcinoma cell lines
